| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | WT6; XBR; NRSF |
| WB Predicted band size | 122 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human REST |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于REST(RE1沉默转录因子，也称NRSF)抗体的3篇关键文献及摘要概括：
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1. **文献名称**：*REST: A mammalian silencer protein that restricts sodium channel gene expression to neurons*
**作者**：Schoenherr, C.J., & Anderson, D.J.
**摘要**：该研究首次鉴定REST蛋白作为神经元基因的抑制因子，通过结合神经元特异性基因的RE1沉默元件，限制其在非神经元细胞中的表达，为后续研究REST在神经分化中的作用奠定了基础。
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2. **文献名称**：*REST regulates a transcriptional program for neuronal differentiation through epigenetic modification*
**作者**：Ballas, N., et al.
**摘要**：本文揭示了REST通过招募组蛋白修饰复合物(如HDAC和MeCP2)，在神经分化过程中动态调控染色质状态，从而抑制非神经元细胞中神经元基因的表达，强调了其表观遗传调控机制。
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3. **文献名称**：*REST and its corepressors mediate plasticity of neuronal gene chromatin throughout neurogenesis*
**作者**：Sun, Y., et al.
**摘要**：研究证明REST与共抑制因子(如CoREST)协同作用，在神经发生过程中动态调节神经元基因的染色质可塑性，并发现REST缺失会导致胚胎致死，进一步阐明其在发育中的关键作用。
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4. **文献名称**：*The neuron-restrictive silencer factor (NRSF/REST) controls survival and secretion of Alzheimer’s disease amyloid-β peptide precursor*
**作者**：Lu, T., et al.
**摘要**：该文献报道了REST在阿尔茨海默病中的保护作用，指出REST通过抑制β-淀粉样前体蛋白(APP)基因的表达，减少毒性Aβ肽的产生，并发现其在老年患者脑组织中表达降低，与认知衰退相关。
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这些文献涵盖了REST的核心功能、表观调控机制及疾病关联，为抗体应用提供理论支持。如需具体实验方法学文献，可进一步补充。
RE-1 Silencing Transcription factor (REST), also known as Neuron-Restrictive Silencing Factor (NRSF), is a transcriptional repressor that regulates the expression of neuronal genes in non-neuronal cells and neural progenitor cells. Discovered in 1995. REST binds to a conserved DNA motif called the RE1/NRSE (Neuron-Restrictive Silencing Element) to recruit co-repressor complexes, including histone deacetylases (HDACs) and methyltransferases, which modify chromatin structure to suppress gene transcription. This mechanism ensures neuronal-specific genes remain silenced outside the nervous system, maintaining cellular identity.
REST plays critical roles in neurodevelopment, stem cell pluripotency, and cancer. During neural differentiation, REST levels decline, allowing activation of genes essential for neuronal function. Dysregulation of REST is linked to neurological disorders (e.g., epilepsy, Alzheimer’s) and cancers, where it can act as either an oncogene or tumor suppressor depending on context. In tumors like neuroblastoma or glioblastoma, aberrant REST activity may promote proliferation or resistance to therapy.
Antibodies targeting REST are vital tools for studying its expression, localization, and interactions. They enable detection of REST in tissues or cell lines via techniques like Western blot, immunohistochemistry, and ChIP-seq. Researchers also use REST antibodies to explore post-translational modifications (e.g., phosphorylation, ubiquitination) that modulate its stability and activity. Understanding REST's dual roles in health and disease continues to drive therapeutic innovation, particularly in targeting REST-associated pathways in cancer and neurodegeneration.
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