首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：犬胃泌素(GAST)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GAST含量。GAST是胃泌素基因，胃泌素作为胃肠激素，可调节胃酸分泌、促进胃肠道黏膜生长等。其研究机制主要围绕在消化系统疾病如胃溃疡、胃癌等方面，探究其在疾病发生发展中的作用，以及通过调控GAST来开发相关治疗方法。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于科研领域中对犬科动物胃肠疾病模型构建、营养代谢研究、药物干预效果评估等实验需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GAST ELISA Kit; GASGastrin [Cleaved into: Big gastrin ELISA Kit; Gastrin-34 ELISA Kit; G34); Gastrin] ELISA Kit
缩写：GAST
Uniprot No.：P01353
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog GAST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 81-88 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 83-96 | |||||
回收率：
| The recovery of dog GAST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-96 | ||||
| EDTA plasma (n=4) | 89 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4001.9981.8831.9411.8142001.4631.3681.4161.2891000.9561.0400.9980.871500.6230.6550.6390.512250.4220.4260.4240.29712.50.2870.2700.2790.1526.250.1900.2020.1960.06900.1320.1220.127 | ||||||
货期：3-5 working days
×
