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Rabbit Polyclonal CHFR Antibody

  • 中文名: CHFR抗体
  • 别    名: RNF116; RNF196
货号: IPDX13137
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/20-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesRNF116; RNF196
WB Predicted band size73 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenSynthetic peptide of human CHFR
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于CHFR抗体的3篇代表性文献,涵盖其在癌症研究中的应用及机制探索:

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1. **文献名称**:*CHFR is required for tumour suppression and Aurora A regulation*

**作者**:Scolnick, D.M., Halverson, D.

**摘要**:该研究利用CHFR抗体进行免疫印迹分析,揭示了CHFR蛋白在有丝分裂检查点中的关键作用,并发现其通过调控Aurora A激酶活性维持基因组稳定性,为肿瘤抑制机制提供了新见解。

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2. **文献名称**:*Frequent epigenetic inactivation of CHFR in gastric carcinoma*

**作者**:Suzuki, H., et al.

**摘要**:通过甲基化特异性PCR和CHFR抗体免疫组化分析,研究发现胃癌中CHFR启动子高频甲基化导致蛋白表达缺失,且与患者不良预后相关,提示CHFR作为潜在的诊断标志物。

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3. **文献名称**:*CHFR promoter methylation correlates with prognosis in non-small cell lung cancer*

**作者**:Koga, Y., et al.

**摘要**:该研究在非小细胞肺癌中发现CHFR启动子甲基化与蛋白表达降低(通过抗体检测)显著相关,且甲基化状态可作为独立预后因素,为靶向治疗提供了理论依据。

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4. **文献名称**:*Loss of CHFR expression in colorectal cancer: a biomarker of aggressive tumors*

**作者**:Karra, H., et al.

**摘要**:利用CHFR抗体进行免疫组织化学分析,研究显示结直肠癌中CHFR蛋白表达缺失与肿瘤侵袭性增强及总生存期缩短相关,支持其作为临床预后评估工具的价值。

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这些文献均通过CHFR抗体探究了该蛋白在癌症中的表达模式及临床意义,涵盖机制研究与转化医学应用。

背景信息

The CHFR (Checkpoint with FHA and RING Domains) protein is a critical regulator of the cell cycle checkpoint response, particularly under conditions of mitotic stress such as microtubule disruption. It functions as an E3 ubiquitin ligase, leveraging its RING domain to mediate ubiquitination and its FHA domain to interact with phosphorylated targets. CHFR delays mitotic entry by targeting proteins like PLK1 for degradation, providing time for DNA repair or stress resolution. Dysregulation of CHFR is implicated in cancer, where promoter hypermethylation silences its expression, correlating with tumor progression, chemoresistance (e.g., to taxanes), and poor prognosis in colorectal, lung, and gastric cancers. CHFR antibodies are essential tools for detecting its expression or loss in research, enabling studies on epigenetic silencing mechanisms, biomarker potential, and therapeutic targeting. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore CHFR's role in maintaining genomic stability and its clinical relevance in oncology.

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