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产品描述:人干扰素诱导鸟苷酸结合蛋白1(GBP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GBP1含量。试剂盒检测范围为25 pg/mL-1600 pg/mL,本试剂盒适用于基础科研领域,例如探究病原体感染后宿主免疫反应、炎症信号通路调控、肿瘤免疫逃逸机制等研究场景,为体外细胞实验或动物模型中GBP1的功能研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:GBP 1 ELISA Kit; GBP-1 ELISA Kit; GBP1 ELISA Kit; GBP1_HUMAN ELISA Kit; GTP binding protein 1 ELISA Kit; GTP-binding protein 1 ELISA Kit; Guanine nucleotide binding protein 1 ELISA Kit; Guanine nucleotide-binding protein 1 ELISA Kit; Guanylate binding protein 1 ELISA Kit; Guanylate binding protein 1 interferon inducible 67kDa ELISA Kit; Guanylate binding protein 1 interferon inducible ELISA Kit; HuGBP 1 ELISA Kit; HuGBP-1 ELISA Kit; HuGBP1 ELISA Kit; Interferon induced guanylate binding protein 1 ELISA Kit; Interferon-induced guanylate-binding protein 1 ELISA Kit; OTTHUMP00000012352 ELISA Kit
缩写:GBP1
Uniprot No.:P32455
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates, cell lysates
检测范围:25 pg/mL-1600 pg/mL
灵敏度:6.25 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human GBP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 86 | ||||
Range % | 82-92 | |||||
1:2 | Average % | 98 | ||||
Range % | 94-105 | |||||
1:4 | Average % | 97 | ||||
Range % | 93-110 | |||||
1:8 | Average % | 94 | ||||
Range % | 87-98 |
回收率:
The recovery of human GBP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 90-98 | ||||
EDTA plasma (n=4) | 98 | 91-100 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pg/mlOD1OD2AverageCorrected16001.7941.9521.8731.7788001.1751.1011.1381.0434000.6340.6040.6190.5242000.3510.3390.3450.2501000.2750.2700.2730.178500.1950.1920.1940.099250.1420.1400.1410.04600.0950.0940.095 |
货期:3-5 working days
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