| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | LKLF |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human KLF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与KLF2抗体相关的文献摘要简述：
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1. **文献名称**：*Krüppel-like factor 2 regulates T cell trafficking and activation in immunity*
**作者**：Carlson, C.M.等
**摘要**：研究证明KLF2通过调控T细胞迁移相关受体(如S1P1)的表达，影响T细胞的存活和归巢。文中使用KLF2抗体进行Western blot和流式细胞术，验证KLF2蛋白在T细胞中的表达下调对免疫功能的影响。
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2. **文献名称**：*The endothelial transcription factor KLF2 regulates vascular homeostasis through distinct protein interaction networks*
**作者**：Atkins, G.B.等
**摘要**：探讨KLF2在内皮细胞稳态中的作用，发现其通过调控抗炎和抗血栓形成基因的表达维持血管功能。研究采用KLF2抗体进行染色质免疫共沉淀(ChIP)和免疫荧光，揭示KLF2与靶基因启动子的结合机制。
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3. **文献名称**：*KLF2 suppresses macrophage inflammatory response through transcriptional regulation of NF-κB signaling*
**作者**：Das, H.等
**摘要**：揭示KLF2通过抑制NF-κB通路减轻巨噬细胞炎症反应。实验通过KLF2抗体进行免疫组化，证实KLF2高表达与炎症因子(如TNF-α、IL-6)的负相关性，为抗炎治疗提供新靶点。
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以上文献均利用KLF2抗体作为关键实验工具，涵盖免疫调控、血管稳态及炎症机制等研究方向。如需具体DOI或发表年份，可进一步补充检索。
KLF2 (Krüppel-like factor 2) is a transcription factor belonging to the Krüppel-like family, characterized by C-terminal zinc finger domains that bind GC-rich DNA sequences. It regulates diverse biological processes, including cell differentiation, proliferation, inflammation, and vascular homeostasis. KLF2 is highly expressed in endothelial cells, T lymphocytes, and adipocytes, where it modulates genes involved in redox balance, apoptosis, and immune responses. For example, in endothelial cells, KLF2 maintains vascular integrity by suppressing pro-inflammatory pathways, while in T cells, it promotes a quiescent state and regulates trafficking.
KLF2 antibodies are immunological tools used to detect and quantify KLF2 protein levels in research applications such as Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and immunohistochemistry. These antibodies are critical for studying KLF2's role in development and disease, including atherosclerosis, cancer metastasis, and autoimmune disorders. Commercially available KLF2 antibodies are typically raised in rabbits or mice using immunogenic peptides derived from conserved regions of human or mouse KLF2. Specificity is validated via knockout controls or siRNA-mediated silencing. Researchers rely on these antibodies to explore KLF2's interactions with co-regulators (e.g., HDACs) and its downstream targets (e.g., eNOS, NF-κB), offering insights into therapeutic strategies for vascular and immune-related pathologies.
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