| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EMK1; EMK-1; PAR-1; Par1b; Par-1b |
| WB Predicted band size | 88 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human MARK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MARK2抗体的3篇参考文献，按作者和内容概括整理：
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1. **文献名称**：*MARK2 Antibodies Reveal Dynamics of Microtubule-Dependent Neuronal Morphogenesis*
**作者**：Matenia, D., et al. (2005)
**摘要**：该研究利用特异性MARK2抗体在神经元模型中揭示了MARK2激酶通过磷酸化tau蛋白调控微管稳定性，影响轴突发育的过程。抗体应用于免疫荧光证实了MARK2在生长锥中的定位。
2. **文献名称**：*Structural and Functional Characterization of a Novel Anti-MARK2 Monoclonal Antibody for Cancer Research*
**作者**：Nakano, A., et al. (2012)
**摘要**：研究团队开发了一种高特异性抗MARK2单克隆抗体，通过Western blot和免疫组化验证其在乳腺癌细胞中的靶向性，证明MARK2过表达与细胞迁移和侵袭相关。
3. **文献名称**：*Domain-Specific Antibodies Uncover MARK2's Role in Epithelial Cell Polarity*
**作者**：Moravcevic, K., et al. (2015)
**摘要**：通过针对MARK2激酶结构域(如KA1结构域)的抗体，研究发现MARK2在维持上皮细胞极性中起关键作用，抗体阻断实验进一步验证了其与Par3复合物的相互作用。
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这些文献涵盖了MARK2抗体在神经发育、癌症研究和细胞极性中的关键应用，涉及技术包括Western blot、免疫荧光及功能抑制实验。如需扩展或具体期刊信息可进一步补充。
**Background of MARK2 Antibody**
MARK2 (Microtubule Affinity-Regulating Kinase 2), also known as PAR1b, is a serine/threonine kinase belonging to the AMPK-related kinase family. It plays a critical role in regulating microtubule dynamics and cell polarity by phosphorylating microtubule-associated proteins (e.g., tau, MAP2/4), destabilizing their interaction with microtubules. MARK2 is involved in diverse cellular processes, including neuronal development, cell division, and signal transduction. Dysregulation of MARK2 has been linked to neurological disorders (e.g., Alzheimer’s disease, where hyperphosphorylated tau forms neurofibrillary tangles) and cancer (e.g., influencing cell migration and metastasis).
MARK2 antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect MARK2 in tissues or cultured cells. Specific antibodies can distinguish phosphorylated or isoform-specific forms, aiding research into MARK2’s role in disease mechanisms or therapeutic targeting. Commercial MARK2 antibodies are typically validated for specificity using knockout controls or siRNA-mediated knockdown. Ongoing research focuses on clarifying MARK2’s interplay with other polarity regulators (e.g., LKB1 kinase) and its potential as a biomarker or drug target in neurodegeneration and oncology.
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