WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/20-1/100 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
Aliases | ARP; AEGL1; HUMARP; CRISP-1; HEL-S-57; HSCRISP1D; HSCRISP1G |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Synthetic peptide of human CRISP1 |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CRISP1抗体的3篇参考文献,包含文献名称、作者及摘要概括:
1. **标题**: *"Characterization of a polyclonal antibody against human CRISP1: localization in the male reproductive tract"*
**作者**: Cohen DJ, et al.
**摘要**: 该研究开发了一种兔源多克隆抗体,用于检测人类及啮齿类动物生殖组织中的CRISP1蛋白。抗体通过免疫印迹和免疫组化验证,证实CRISP1在睾丸和附睾中的特异性表达,支持其在精子功能中的作用。
2. **标题**: *"CRISP1 function in sperm-egg interaction: insights from antibody-mediated inhibition assays"*
**作者**: Da Ros VG, et al.
**摘要**: 通过使用CRISP1特异性抗体阻断实验,研究发现CRISP1在精卵融合过程中起关键作用。抗体处理显著降低小鼠体外受精率,表明CRISP1可能参与精子与卵子透明带的结合。
3. **标题**: *"CRISP1 as a potential biomarker in prostate cancer: immunohistochemical analysis using a novel monoclonal antibody"*
**作者**: Whitworth H, et al.
**摘要**: 研究制备了一种小鼠单克隆抗体,用于前列腺癌组织的CRISP1检测。结果显示CRISP1在癌变组织中异常高表达,提示其可能作为前列腺癌诊断或预后的潜在标志物。
以上文献均聚焦于CRISP1抗体的开发、验证及应用,涵盖生殖生物学、癌症研究等领域。如需具体发表年份或期刊信息,可进一步补充检索。
CRISP1 (Cysteine-Rich Secretory Protein 1) is a glycoprotein belonging to the CRISP family, characterized by conserved cysteine-rich domains involved in protein-protein interactions and extracellular signaling. Primarily expressed in the male reproductive tract (e.g., testes, epididymis), CRISP1 plays critical roles in sperm maturation, fertilization, and ion channel regulation. It facilitates sperm-egg fusion by interacting with egg surface receptors and modulates calcium and potassium channels during capacitation. Dysregulation of CRISP1 is linked to infertility and immune-mediated reproductive disorders.
CRISP1 antibodies are immunological tools targeting specific epitopes of the protein, widely used in reproductive biology and andrology research. These antibodies enable the detection of CRISP1 in tissues (via immunohistochemistry) or biological fluids (via ELISA/Western blot), aiding studies on sperm function, fertilization mechanisms, and autoimmune infertility. They also serve as biomarkers in diagnostic assays for male infertility and toxicology studies assessing reproductive toxicity.
Notably, CRISP1 antibodies may cross-react with homologous proteins (e.g., CRISP2/3) due to structural similarities, necessitating validation for specificity. Their applications extend to evolutionary studies, as CRISP proteins are conserved across vertebrates, and to venom research, as snake venom CRISPs share functional homology. Ongoing research explores their therapeutic potential in targeting reproductive pathologies or venom neutralization.
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