首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠果糖-6-磷酸异构酶(GPI)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GPI含量。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于基础科研中能量代谢相关模型构建、药物干预效应评估及分子机制探索等研究场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gpi ELISA Kit; Gpi1Glucose-6-phosphate isomerase ELISA Kit; GPI ELISA Kit; EC 5.3.1.9 ELISA Kit; Autocrine motility factor ELISA Kit; AMF ELISA Kit; Neuroleukin ELISA Kit; NLK ELISA Kit; Phosphoglucose isomerase ELISA Kit; PGI ELISA Kit; Phosphohexose isomerase ELISA Kit; PHI ELISA Kit
缩写：GPI
Uniprot No.：P06745
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 90-99 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 87-98 | ||||||
| 1:4 | Average % | 100 | |||||
| Range % | 94-105 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 91-102 | ||||||
回收率：
| The recovery of mouse GPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 89-102 | |||||
| EDTA plasma (n=4) | 102 | 95-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 502.5382.4222.4802.308 251.7361.6841.7101.538 12.51.1971.1431.1700.998 6.250.6890.6810.6850.513 3.120.4140.3890.4020.230 1.560.2950.2850.2900.118 0.780.2230.2260.2250.053 00.1740.1700.172 | |||||||
货期：3-5 working days
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