| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SH3GLB1 |
| Uniprot No | Q9Y371 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-365aa |
| 氨基酸序列 | MNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAE CTKIWTEKIMKQTEVLLQPNPNARIEEFVYEKLDRKAPSRINNPELLGQY MIDAGTEFGPGTAYGNALIKCGETQKRIGTADRELIQTSALNFLTPLRNF IEGDYKTIAKERKLLQNKRLDLDAAKTRLKKAKAAETRNSSEQELRITQS EFDRQAEITRLLLEGISSTHAHHLRCLNDFVEAQMTYYAQCYQYMLDLQK QLGSFPSNYLSNNNQTSVTPVPSVLPNAIGSSAMASTSGLVITSPSNLSD LKECSGSRKARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERG NQKGKVPITYLELLNLEHHHHHH |
| 预测分子量 | 42 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SH3GLB1重组蛋白的3篇代表性文献，按作者、标题和摘要内容概括整理：
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1. **文献名称**：*SH3GLB1/Bif-1 regulates mitochondrial dynamics and metabolic reprogramming in cancer cells*
**作者**：Takahashi Y, et al.
**摘要**：
该研究通过表达纯化SH3GLB1重组蛋白，揭示其通过调控线粒体分裂蛋白Drp1的活性，影响线粒体形态和细胞代谢重编程，从而促进肿瘤细胞存活。实验表明SH3GLB1缺失会导致线粒体过度融合并抑制糖酵解。
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2. **文献名称**：*Structural basis of SH3GLB1 pro-apoptotic function in the BCL-2 pathway*
**作者**：Cuddeback SM, et al.
**摘要**：
本研究解析了重组SH3GLB1蛋白的晶体结构，发现其SH3结构域通过特异性结合BAX蛋白的促凋亡表位，激活线粒体凋亡通路。体外实验显示重组SH3GLB1可增强BAX介导的细胞色素C释放。
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3. **文献名称**：*Recombinant SH3GLB1 induces autophagy via PI3K/AKT/mTOR pathway inhibition*
**作者**：Wang L, et al.
**摘要**：
利用大肠杆菌表达系统制备功能性SH3GLB1重组蛋白，证明其通过抑制PI3K/AKT/mTOR信号通路促进自噬小体形成。该蛋白在神经退行性疾病模型中显示出减少错误折叠蛋白聚集的潜力。
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**备注**：若需具体文献年份或DOI，建议通过PubMed/Google Scholar搜索标题进一步获取。部分研究可能侧重SH3GLB1的基因功能而非重组蛋白制备，筛选时需注意方法学部分描述。
SH3GLB1 (SH3 domain-containing GRB2-like protein B1), also known as endophilin B1 or Bax-interacting factor 1 (Bif-1), is a multifunctional protein involved in membrane dynamics, intracellular trafficking, and apoptosis regulation. It belongs to the endophilin protein family, characterized by an N-terminal BAR (Bin/Amphiphysin/Rvs) domain that binds curved membranes and a C-terminal SH3 domain mediating protein-protein interactions. SH3GLB1 plays critical roles in autophagy, mitochondrial morphology maintenance, and endocytosis by facilitating membrane curvature and recruiting downstream effectors.
This protein interacts with components of the apoptotic machinery, including Bcl-2 family members, and participates in the formation of autophagosomes through its association with the autophagy-related protein Atg9. Its involvement in mitochondrial fission-fusion dynamics links it to cellular energy homeostasis and stress responses. Dysregulation of SH3GLB1 has been implicated in various pathological conditions, including cancer (where it may act as tumor suppressor or promoter depending on context), neurodegenerative diseases, and metabolic disorders.
Recombinant SH3GLB1 proteins are engineered for functional studies, typically expressed in bacterial (E. coli) or mammalian systems with purification tags (e.g., His-tag). These recombinant tools enable researchers to investigate SH3GLB1's structural properties, binding partners, and enzymatic activities in vitro. They are particularly valuable for studying membrane remodeling mechanisms, screening small molecule modulators, and deciphering molecular pathways involving autophagy-apoptosis crosstalk. Recent applications extend to exploring therapeutic strategies targeting membrane trafficking defects and mitochondrial dysfunction-related diseases. The protein's conserved nature across eukaryotes enhances its translational research relevance in both basic and applied biomedical studies.
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