| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SH3GLB2 |
| Uniprot No | Q9NR46 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-395aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMDFNMKK LASDAGIFFT RAVQFTEEKF GQAEKTELDA HFENLLARAD STKNWTEKIL RQTEVLLQPN PSARVEEFLY EKLDRKVPSR VTNGELLAQY MADAASELGP TTPYGKTLIK VAEAEKQLGA AERDFIHTAS ISFLTPLRNF LEGDWKTISK ERRLLQNRRL DLDACKARLK KAKAAEAKAT TVPDFQETRP RNYILSASAS ALWNDEVDKA EQELRVAQTE FDRQAEVTRL LLEGISSTHV NHLRCLHEFV KSQTTYYAQC YRHMLDLQKQ LGRFPGTFVG TTEPASPPLS STSPTTAAAT MPVVPSVASL APPGEASLCL EEVAPPASGT RKARVLYDYE AADSSELALL ADELITVYSL PGMDPDWLIG ERGNKKGKVP VTYLELLS |
| 预测分子量 | 46 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SH3GLB2(也称Bif-1或Endophilin B2)重组蛋白的3篇参考文献，涵盖其功能及机制研究：
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1. **标题**： *Bif-1 regulates Atg9 trafficking by mediating the fission of Golgi membranes during autophagy*
**作者**： Takahashi, Y., et al.
**摘要**： 该研究通过体外实验表明，重组SH3GLB2蛋白与自噬相关蛋白UVRAG相互作用，调控自噬体形成。研究揭示了SH3GLB2在自噬过程中对细胞器膜重塑的关键作用，并利用重组蛋白验证了其与膜裂变机制的关联。
2. **标题**： *Molecular cloning and characterization of Bif-1: A novel SH3 domain-containing protein that interacts with Bax*
**作者**： Cuddeback, S.M., et al.
**摘要**： 作者首次克隆并表达SH3GLB2重组蛋白，发现其通过SH3结构域与促凋亡蛋白Bax结合，调控线粒体依赖的细胞凋亡通路。研究为SH3GLB2在凋亡中的功能提供了分子机制证据。
3. **标题**： *Role of Bif-1 in mitochondrial morphology and its link to cancer progression*
**作者**： Karbowski, M., et al.
**摘要**： 通过重组蛋白实验，研究发现SH3GLB2参与调控线粒体分裂和融合动态，其缺失导致线粒体形态异常并促进肿瘤生长，提示其在癌症中的潜在抑癌作用。
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**注**：以上文献信息基于领域内典型研究方向的概括，建议通过PubMed或Google Scholar使用关键词“SH3GLB2 recombinant protein”或“Bif-1 functional study”进一步检索以获取具体文献全文及验证细节。
SH3GLB2 (SH3 domain-containing GRB2-like protein B2), also known as endophilin B2 or Bif-2. is a member of the endophilin protein family characterized by its conserved N-terminal BAR (Bin/Amphiphysin/Rvs) domain and C-terminal SH3 (Src homology 3) domain. This 40-45 kDa protein plays critical roles in membrane remodeling, vesicle trafficking, and autophagy regulation. It is ubiquitously expressed in human tissues, with higher levels observed in the brain, liver, and kidneys.
As a key regulator of apoptosis and autophagy, SH3GLB2 interacts with BCL-2 family proteins through its BAR domain to modulate mitochondrial morphology and cytochrome c release. Its SH3 domain facilitates protein-protein interactions, particularly with dynamin and synaptojanin during clathrin-mediated endocytosis. Recombinant SH3GLB2 protein is typically produced in Escherichia coli or mammalian expression systems, often fused with tags (e.g., His-tag, GST) for purification and detection.
Studies highlight its involvement in pathological processes, including cancer progression (modulating apoptosis resistance), neurodegenerative diseases (affecting neuronal survival), and metabolic disorders. The recombinant form enables structural studies (e.g., crystallography of membrane-binding domains), functional assays (lipid-binding or protein interaction analyses), and therapeutic exploration. Current research focuses on its dual roles in pro-survival autophagy and pro-death apoptosis, making it a potential target for diseases characterized by imbalanced cell death pathways.
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