首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠免疫球蛋白A(IgA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的IgA含量。IgA是一种免疫球蛋白，主要存在于黏膜表面，具有抗菌和抗病毒作用。IgA肾病是一种以IgA沉积在肾小球系膜区为特征的肾小球疾病，其发病机制复杂，涉及遗传、环境因素和免疫反应等多重打击假说。目前，针对IgA肾病的研究主要集中在靶向B细胞免疫通路、补体系统等靶点，以开发更有效的治疗药物。试剂盒检测范围为25 ng/mL-1600 ng/mL，肠道菌群研究、呼吸道感染模型评估、免疫调节剂药效分析、小鼠感染性疾病模型、自身免疫疾病研究、过敏反应实验中黏膜免疫水平的动态监测、免疫机制探索、生物标志物分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgA
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：25 ng/mL-1600 ng/mL
灵敏度：25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5000 | Average % | 93 | ||||
| Range % | 88-95 | |||||
| 1:10000 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:20000 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:40000 | Average % | 97 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of mouse IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 102 | 98-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 16000.3520.3750.364 4000.4900.4840.487 2000.6180.6610.640 1000.7860.7720.779 251.0051.0881.047 01.8341.9001.867 | ||||||
货期：3-5 working days
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