首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子免疫球蛋白A(IgA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IgA含量。IgA即免疫球蛋白A，是人体黏膜免疫的主要抗体，在抵御病原体入侵、维持黏膜稳态等方面有重要作用。其研究机制主要聚焦于IgA与抗原的识别结合、参与免疫调节过程，以及在黏膜相关疾病如感染性疾病、自身免疫病中的作用机制。试剂盒检测范围为12 ng/mL-3000 ng/mL，尤其适用于疫苗研究、肠道菌群互作实验或慢性炎症模型中黏膜免疫效能的评估，也可用于体外细胞实验探究免疫调节因子对IgA分泌的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgA
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：12 ng/mL-3000 ng/mL
灵敏度：3 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 89 | ||||
| Range % | 84-97 | |||||
| 1:2000 | Average % | 91 | ||||
| Range % | 89-96 | |||||
| 1:4000 | Average % | 93 | ||||
| Range % | 87-104 | |||||
| 1:8000 | Average % | 92 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rabbit IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 88-103 | ||||
| EDTA plasma (n=4) | 95 | 85-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average30000.2210.2180.2207500.3510.3540.353187.50.6290.6250.627471.4641.5021.483122.2592.2352.24703.2183.1553.187 | ||||||
货期：3-5 working days
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