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产品描述：猪免疫球蛋白A(IgA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的IgA含量。IgA是免疫球蛋白A，是机体黏膜免疫的主要抗体，在抵御病原体入侵上发挥重要作用。研究机制上，主要聚焦于其与各类疾病的关联，如感染性疾病、自身免疫病等，通过研究其产生、功能及调节机制，来探寻疾病防治新策略。试剂盒检测范围为0.146 μg/mL-37.5 μg/mL，本试剂盒适用于科研领域对猪黏膜免疫功能的评估研究。该产品适用于畜牧科研中疫苗效力评估、疾病模型建立时的免疫应答监测，或规模化养殖中通过定期检测 IgA 水平分析猪群健康状态。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgA
种属：Sus scrofa (Pig)
样本类型：serum, plasma
检测范围：0.146 μg/mL-37.5 μg/mL
灵敏度：0.146 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 92 | ||||
| Range % | 88-94 | |||||
| 1:2000 | Average % | 93 | ||||
| Range % | 90-98 | |||||
| 1:4000 | Average % | 88 | ||||
| Range % | 85-94 | |||||
| 1:8000 | Average % | 95 | ||||
| Range % | 91-100 | |||||
回收率：
| The recovery of pig IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 103 | 98-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 37.50.1990.2090.204 9.3750.4450.4200.433 2.3450.9240.9550.940 0.5861.4131.4471.430 0.1462.1012.1142.108 02.5472.5032.525 | ||||||
货期：3-5 working days
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