| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMCP |
| Uniprot No | P49901 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-116aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMCDQTKH SKCCPAKGNQ CCPPQQNQCC QSKGNQCCPP KQNQCCQPKG SQCCPPKHNH CCQPKPPCCI QARCCGLETK PEVSPLNMES EPNSPQTQDK GCQTQQQPHS PQNESRPSK |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SMCP(精子线粒体相关半胱氨酸丰富蛋白)重组蛋白研究的3篇示例文献摘要(注：文献为假设性示例，实际引用需核实具体来源)：
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1. **文献名称**: *Expression and Functional Analysis of Recombinant Sperm Mitochondrial Cysteine-rich Protein (SMCP) in Bovine*
**作者**: Hess RA, et al.
**摘要**: 本研究在大肠杆菌系统中成功表达重组牛SMCP蛋白，并验证其在精子线粒体鞘结构中的作用。实验表明，重组SMCP可结合线粒体膜蛋白，提示其参与精子运动能量代谢的调控。
2. **文献名称**: *Production of Polyclonal Antibodies Using Recombinant SMCP for Sperm Maturation Studies*
**作者**: Flickinger CJ, et al.
**摘要**: 通过重组小鼠SMCP蛋白免疫兔，获得高特异性抗体。免疫组化显示SMCP在精子成熟过程中向线粒体鞘的迁移定位，为精子功能异常提供了分子标记。
3. **文献名称**: *SMCP Interaction with Mitochondrial Proteins: A Recombinant Protein-Based Binding Assay*
**作者**: King SM, Insdorf NF
**摘要**: 利用重组人SMCP进行体外结合实验，发现其与COX6B2等线粒体蛋白存在直接互作，揭示了SMCP在精子运动调控中的潜在分子机制。
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如需真实文献，建议通过PubMed或Web of Science检索关键词“SMCP recombinant protein”或“sperm mitochondrial cysteine-rich protein expression”获取最新研究。
SMCP (Sperm Mitochondria-associated Cysteine-rich Protein) is a cysteine-rich structural protein predominantly expressed in developing male germ cells. It plays a critical role in the organization and stability of sperm mitochondrial sheaths during spermatogenesis. Encoded by the *Smcp* gene, SMCP is localized to the mitochondrial capsule in spermatozoa, where it contributes to the unique architecture of sperm mitochondria by cross-linking with other structural proteins. Its cysteine-rich domains facilitate disulfide bond formation, essential for maintaining mitochondrial integrity under the mechanical stress of sperm motility.
Dysregulation or mutations in SMCP have been linked to sperm morphological abnormalities and male infertility, particularly asthenozoospermia (reduced sperm motility). This association has driven interest in SMCP as a biomarker for diagnosing male reproductive disorders and a potential target for therapeutic interventions.
Recombinant SMCP proteins are typically produced using bacterial (e.g., *E. coli*) or mammalian expression systems, enabling large-scale purification for research and clinical applications. These engineered proteins retain the native structure and functional properties required for studying SMCP’s biological roles, including mitochondrial dynamics, energy metabolism, and sperm motility mechanisms. In reproductive biology, recombinant SMCP serves as a tool to investigate molecular pathways in spermatogenesis and to develop antibody-based assays for fertility testing. Additionally, it holds promise in contraceptive vaccine development by targeting sperm-specific antigens.
The production of recombinant SMCP also supports drug discovery efforts aimed at addressing mitochondrial dysfunction in sperm cells. Its application extends to comparative studies across species, aiding evolutionary insights into sperm adaptation. Overall, SMCP recombinant proteins bridge basic research and translational medicine in andrology and reproductive health.
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