| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNRPB2 |
| Uniprot No | P08579 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-225aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMDIRPNHTIYINNMNDKIKKEELKRSLYAL FSQFGHVVDIVALKTMKMRGQAFVIFKELGSSTNALRQLQGFPFYGKPMR IQYAKTDSDIISKMRGTFADKEKKKEKKKAKTVEQTATTTNKKPGQGTPN SANTQGNSTPNPQVPDYPPNYILFLNNLPEETNEMMLSMLFNQFPGFKEV RLVPGRHDIAFVEFENDGQAGAARDALQGFKITPSHAMKITYAKK |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNRPB2重组蛋白的3篇参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*Structural Insights into SNRPB2 in Spliceosome Assembly*
**作者**：Zhang Y, et al.
**摘要**：本研究利用重组表达的SNRPB2蛋白，通过X射线晶体学解析其三维结构，揭示了其与U1 snRNA结合的关键结构域，并探讨其在剪接体早期组装中的功能机制。
2. **文献名称**：*SNRPB2 Recombinant Protein Interacts with SMN Complex in Spinal Muscular Atrophy Models*
**作者**：Li H, et al.
**摘要**：通过体外表达纯化的SNRPB2重组蛋白，作者证明了其与SMN蛋白复合物的相互作用，并发现SNRPB2异常定位可能导致脊髓性肌萎缩症的剪接缺陷。
3. **文献名称**：*Functional Analysis of SNRPB2 Phosphorylation in Cancer Cell Splicing*
**作者**：Wang X, et al.
**摘要**：研究构建了SNRPB2磷酸化位点突变的重组蛋白，结合RNA-seq分析，揭示了其在肺癌细胞中调控选择性剪接的分子机制及促癌作用。
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建议通过PubMed、Google Scholar等平台以“SNRPB2 recombinant protein”或“SNRPB2 expression and function”为关键词检索最新文献，并优先选择结构生物学、剪接机制或疾病模型相关的高影响力期刊论文。
SNRPB2 (Small Nuclear Ribonucleoprotein Polypeptide B2) is a core component of the spliceosome, a macromolecular complex responsible for pre-mRNA splicing in eukaryotic cells. It belongs to the Sm protein family, which plays a critical role in recognizing and binding specific sites on spliceosomal small nuclear RNAs (snRNAs) to assemble small nuclear ribonucleoprotein (snRNP) particles. These particles are essential for the accurate removal of introns and ligation of exons during mRNA processing. SNRPB2 interacts with other spliceosomal proteins, such as SNRPB and SNRPA, forming a heteromeric Sm core that stabilizes snRNA structure and facilitates spliceosome assembly.
Recombinant SNRPB2 protein is produced using biotechnological systems (e.g., E. coli or mammalian cell lines) to express the protein under controlled conditions. Its production often involves cloning the SNRPB2 gene into expression vectors, followed by purification using affinity chromatography or other biochemical methods. The recombinant protein retains functional domains, including conserved Sm motifs that mediate RNA binding and protein-protein interactions. This makes it a valuable tool for studying spliceosome dynamics, RNA-protein interactions, and mechanisms of alternative splicing.
Research applications of recombinant SNRPB2 include in vitro splicing assays, structural studies (e.g., cryo-EM), and investigations into diseases linked to spliceosome dysfunction, such as cancer, spinal muscular atrophy, or autoimmune disorders. Mutations in SNRPB2 or dysregulation of its expression have been implicated in aberrant splicing events contributing to pathogenesis. Additionally, the protein is used to generate antibodies for diagnostic purposes or to develop therapeutic strategies targeting spliceosome-related pathways. Its role in fundamental gene expression processes underscores its importance in both basic and translational research.
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