| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNRPF |
| Uniprot No | P62306 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-86aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSLPLNPKPFLNGLTGKPVMVKLKWGMEYK GYLVSVDGYMNMQLANTEEYIDGALSGHLGEVLIRCNNVL YIRGVEEEEEDGEMRE |
| 预测分子量 | 12 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNRPF重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Structural characterization of the human spliceosomal protein SNRPF*
**作者**：Li S, et al.
**摘要**：研究通过重组表达纯化人源SNRPF蛋白，结合X射线晶体学分析其结构，揭示其参与剪接体组装的锌指结构域特征及与其他剪接因子的相互作用机制。
2. **文献名称**：*Recombinant SNRPF facilitates in vitro spliceosome assembly*
**作者**：Zhang Y, et al.
**摘要**：利用大肠杆菌系统表达SNRPF重组蛋白，验证其在体外剪接体组装中的功能，证明SNRPF对pre-mRNA剪接的催化活性具有关键调控作用。
3. **文献名称**：*SNRPF mutations linked to retinitis pigmentosa disrupt protein stability*
**作者**：Wang H, et al.
**摘要**：通过重组技术构建SNRPF突变体，发现与视网膜色素变性相关的突变导致蛋白稳定性下降，影响剪接体功能，为疾病机制提供分子层面的解释。
(注：以上文献为虚拟示例，实际研究需通过学术数据库检索确认。)
**Background of SNRPF Recombinant Protein**
The Small Nuclear Ribonucleoprotein Polypeptide F (SNRPF) is a core component of the spliceosomal small nuclear ribonucleoprotein (snRNP) complexes, which are essential for pre-mRNA splicing—a critical step in eukaryotic gene expression. SNRPF, along with other Sm proteins (SNRPD1. SNRPD2. SNRPD3. SNRPE, SNRPB, and SNRPG), forms a heptameric ring that binds to uridine-rich regions of spliceosomal small nuclear RNAs (snRNAs), stabilizing their structure and facilitating the assembly of functional spliceosomes. This process ensures the precise removal of introns and ligation of exons, enabling the production of mature mRNA for translation.
Recombinant SNRPF protein is produced through genetic engineering techniques, typically by expressing the SNRPF gene in bacterial or mammalian systems. The purified protein retains its ability to interact with snRNAs and other spliceosomal components, making it a valuable tool for studying spliceosome dynamics, RNA-protein interactions, and splicing mechanisms in vitro. Its applications extend to investigating mutations or dysregulation in snRNP assembly linked to diseases such as retinitis pigmentosa, spinal muscular atrophy, and certain cancers.
Additionally, SNRPF recombinant protein is used in structural studies (e.g., X-ray crystallography or cryo-EM) to resolve spliceosome architecture and in drug discovery to screen for compounds targeting splicing defects. Its role in autoimmune disorders, where anti-Sm antibodies target snRNPs in systemic lupus erythematosus (SLE), further underscores its biomedical relevance. By enabling precise biochemical and functional analyses, SNRPF recombinant protein contributes to both basic research and therapeutic development in RNA biology and related pathologies.
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