| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNRPD2 |
| Uniprot No | P62316 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-118aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MSLLNKPKSE MTPEELQKRE EEEFNTGPLS VLTQSVKNNT QVLINCRNNK KLLGRVKAFD RHCNMVLENV KEMWTEVPKS GKGKKKSKPV NKDRYISKMF LRGDSVIVVL RNPLIAGK |
| 预测分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNRPD2重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*SNRPD2 is a critical mediator of the survival and maintenance of hematopoietic stem cells*
**作者**：Zhang Y, et al.
**摘要**：本研究通过重组SNRPD2蛋白体外功能实验，发现其在造血干细胞中调控RNA剪接过程，并影响细胞存活与增殖。重组蛋白的过表达可抑制DNA损伤诱导的细胞凋亡。
2. **文献名称**：*Structural insights into the assembly and function of the human snRNP core components*
**作者**：Li C, et al.
**摘要**：利用重组SNRPD2蛋白进行X射线晶体学分析，揭示了其与Sm蛋白复合物的互作界面，阐明了其在剪接体核心组装中的关键结构作用。
3. **文献名称**：*Proteomic analysis identifies SNRPD2 as a diagnostic biomarker in hepatocellular carcinoma*
**作者**：Wang X, et al.
**摘要**：通过重组SNRPD2蛋白制备抗体，发现其在肝癌组织中异常高表达，并验证其通过调控致癌基因剪接促进肿瘤进展的分子机制。
注：上述文献为示例性内容，实际研究中建议通过PubMed等数据库以"SNRPD2 recombinant"为关键词检索最新文献。
SNRPD2 (Small Nuclear Ribonucleoprotein D2 Polypeptide) is a core component of the spliceosome, a dynamic macromolecular machinery essential for pre-mRNA splicing in eukaryotic cells. It belongs to the Sm protein family, characterized by a conserved Sm domain that mediates binding to uridine-rich small nuclear RNAs (snRNAs) and facilitates the assembly of snRNP (small nuclear ribonucleoprotein) particles. Specifically, SNRPD2 is a critical subunit of the U1. U2. U4. and U5 snRNPs, which recognize splice sites and catalyze intron removal during mRNA maturation.
Recombinant SNRPD2 protein is produced using expression systems like *E. coli* or mammalian cells, enabling studies of its structural and functional roles in spliceosome dynamics. Its recombinant form retains the ability to interact with other Sm proteins (e.g., SNRPD1. SNRPE) and snRNAs, forming stable snRNP complexes. Researchers utilize it to investigate spliceosome assembly mechanisms, RNA-protein interactions, and splicing regulation in vitro. Dysregulation of SNRPD2 has been linked to diseases, including cancer and neurodegeneration. For example, overexpression of SNRPD2 correlates with poor prognosis in malignancies like breast and colorectal cancer, potentially due to aberrant splicing of oncogenic transcripts. Conversely, reduced levels may impair splicing fidelity, contributing to cellular stress.
Additionally, recombinant SNRPD2 serves as an antigen for antibody development and a tool for screening splicing-modulating compounds. Its conserved structure across eukaryotes makes it a model for studying evolutionary aspects of RNA processing. Ongoing research aims to elucidate its post-translational modifications (e.g., phosphorylation) and non-canonical roles in transcription or telomere maintenance. Understanding SNRPD2’s molecular functions could advance therapies targeting splicing defects in genetic disorders or cancer.
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