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产品描述：猪基质金属蛋白酶9(MMP-9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP-9含量。MMP-9是一种重要靶点。它在机体生理与病理过程中发挥关键作用。在肿瘤中，它能促进肿瘤细胞的侵袭与转移。研究机制多围绕其对细胞外基质的降解、信号通路的调控等方面展开，对其深入研究有助于开发新的治疗策略。试剂盒检测范围为0.625 ng/mL-40 ng/mL，该产品适用于动物模型研究，例如炎症性疾病机制探索、创伤修复过程分析或与MMP - 9相关的病理状态评估，可为科研人员提供稳定可靠的定量数据支持，但不可用于临床诊断或其他非科研用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MMP-9
Uniprot No.：Q2VI03
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 85-106 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 88-105 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of pig MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 87-100 | ||||
| EDTA plasma (n=4) | 97 | 93-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.5052.5512.5282.411202.3132.3282.3212.204101.7861.7791.7831.66651.1121.1311.1221.0052.50.5740.5890.5820.4651.250.3640.3740.3690.2520.6250.2420.2530.2480.13100.1150.1190.117 | ||||||
货期：3-5 working days
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