首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：犬基质金属蛋白酶9/明胶酶B(MMP-9/Gelatinase B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的MMP9含量。MMP9 即基质金属蛋白酶 9，在细胞外基质降解等过程中发挥重要作用。其异常表达与肿瘤侵袭转移、炎症反应、心血管疾病等密切相关。研究机制涉及调控其表达与活性，探索相关信号通路，以开发针对 MMP9 的治疗策略。试剂盒检测范围为12.5 ng/mL-800 ng/mL，的应用场景包括但不限于犬类炎症性疾病模型研究、肿瘤微环境分析、创伤愈合机制探索等基础科研领域，为研究者提供可靠的蛋白表达数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MMP9Matrix metalloproteinase-9 ELISA Kit; MMP-9 ELISA Kit; EC 3.4.24.35 ELISA Kit; 92 kDa gelatinase ELISA Kit; 92 kDa type IV collagenase ELISA Kit; Gelatinase B ELISA Kit; GELB ELISA Kit
缩写：MMP9
Uniprot No.：O18733
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma
检测范围：12.5 ng/mL-800 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 92-105 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 80-96 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 85-90 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-107 | |||||
回收率：
| The recovery of dog MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-89 | ||||
| EDTA plasma (n=4) | 97 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected8002.0962.0762.0861.9774001.5131.6051.5591.4502001.1351.1181.1271.0181000.6180.6550.6370.528500.4230.4090.4160.307250.3010.2780.2900.18112.50.2300.2280.2290.12000.1100.1070.109 | ||||||
货期：3-5 working days
×
