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产品描述：犬髓过氧化物酶(MPO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MPO含量。MPO（髓过氧化物酶）是一种存在于中性粒细胞和单核细胞中的血红素蛋白。它在炎症反应中起关键作用，催化过氧化氢与氯离子反应生成次氯酸等强氧化剂。其异常表达与心血管疾病、神经退行性疾病等多种病理过程相关，研究其机制有助于开发针对性治疗策略。试剂盒检测范围为31.25 ng/mL-2000 ng/mL，科研人员可将其应用于犬类炎症性疾病模型构建、药物抗炎效果评估、免疫调控机制研究等领域，为兽医病理学、比较医学及动物模型研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MPO
Uniprot No.：F1PQ52
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 ng/mL-2000 ng/mL
灵敏度：7.81 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-94 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 97-106 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 87-95 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of dog MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 86-95 | ||||
| EDTA plasma (n=4) | 91 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected20003.0993.1903.1453.04010002.5112.4212.4662.3615001.7871.6111.6991.5942501.0431.0561.0500.9451250.4600.4870.4740.36962.50.2550.2580.2570.15231.250.1550.1600.1580.05300.1020.1080.105 | ||||||
货期：3-5 working days
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