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产品描述：豚鼠基质金属蛋白酶9(MMP-9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP-9含量。MMP - 9是一种基质金属蛋白酶，在细胞外基质降解和重塑中发挥关键作用。其表达异常与肿瘤生长、转移、血管生成以及多种炎症疾病相关。研究机制主要聚焦于其激活调控、底物识别，以及与其他信号通路的相互作用，以探索治疗相关疾病的新策略。试剂盒检测范围为3.12 ng/mL-200 ng/ mL，适用于科研领域中对炎症模型评估、组织再生机制研究或心血管疾病相关实验的定量分析；为豚鼠模型在病理生理机制探索及药物干预效果评价提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MMP-9
Uniprot No.：H0VAD9
种属：Guinea Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/ mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 88-98 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 91-104 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 85-93 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 91-102 | ||||||
回收率：
| The recovery of guinea pig MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 87-97 | |||||
| EDTA plasma (n=4) | 87 | 81-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 2002.2522.1882.2202.071 1001.6351.6761.6561.507 500.9470.9310.9390.790 250.6190.6390.6290.480 12.50.3410.3380.3400.191 6.250.2360.2310.2340.085 3.120.1850.1790.1820.033 00.1500.1480.149 | |||||||
货期：3-5 working days
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