首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠髓过氧化物酶(MPO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MPO含量。MPO（髓过氧化物酶）是一种血红素蛋白，主要存在于中性粒细胞中，参与炎症反应和免疫监视。其研究机制涉及在炎症和免疫过程中催化毒性物质的产生，与多种疾病如炎症性肠病、癌症和心血管疾病相关。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本产品特别适用于科研领域中炎症相关疾病机制研究，如动脉粥样硬化、缺血再灌注损伤、神经退行性疾病等动物模型的炎症标志物分析，亦可用于体外药物筛选或免疫调节实验中MPO活性变化的定量评估，为探索炎症相关通路及干预策略提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mpo ELISA Kit; Myeloperoxidase ELISA Kit; MPO ELISA Kit; EC 1.11.2.2) [Cleaved into: Myeloperoxidase light chain; Myeloperoxidase heavy chain] ELISA Kit
缩写：MPO
Uniprot No.：P11247
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:200 | Average % | 87 | ||||
| Range % | 80-95 | |||||
| 1:400 | Average % | 87 | ||||
| Range % | 85-90 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 89-101 | |||||
回收率：
| The recovery of mouse MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 82-95 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.2932.2332.2632.21210001.3861.2751.3311.2805000.7720.7520.7620.7112500.4960.4910.4940.4431250.2820.2640.2730.22262.50.2220.2050.2140.16331.20.1020.1000.1010.05000.0530.0490.051 | ||||||
货期：3-5 working days
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