首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Noggin蛋白(NOG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的NOG含量。NOG（Noggin）是一种在脊椎动物胚胎发育多个阶段发挥作用的分泌蛋白，参与神经诱导等过程。它通过抑制骨形态发生蛋白（BMP）信号通路来发挥作用，与BMP结合，阻止其与受体结合，从而影响胚胎发育、骨骼和神经系统的形成。NOG在肿瘤机制研究和免疫治疗开发等领域具有潜在应用价值。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，科研人员可将其应用于骨骼发育机制研究、神经干细胞分化模型构建或疾病相关分子通路探索，尤其适用于胚胎发育生物学、再生医学及骨骼疾病动物模型中的NOG动态表达监测，为体外实验或体内模型中蛋白功能研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NogNoggin ELISA Kit
缩写：NOG
Uniprot No.：P97466
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Developmental Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NOG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 95 | |||||
| Range % | 90-97 | ||||||
| 1:10 | Average % | 89 | |||||
| Range % | 81-99 | ||||||
| 1:20 | Average % | 95 | |||||
| Range % | 87-100 | ||||||
| 1:40 | Average % | 93 | |||||
| Range % | 85-104 | ||||||
回收率：
| The recovery of mouse NOG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 88-99 | |||||
| EDTA plasma (n=4) | 97 | 83-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.6012.5932.5972.50510001.4541.4961.4751.3835000.7950.8070.8010.7092500.4320.4260.4290.3371250.2740.2880.2810.18962.50.1810.1750.1780.08631.250.1310.1280.1300.03800.0920.0910.092 | |||||||
货期：3-5 working days
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