首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠骨织素(OSTN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的OSTN含量。OSTN即骨钙素，是一种分泌型骨细胞因子。它在骨骼发育、矿化等过程中发挥重要作用。研究机制上，其能调节成骨细胞与破骨细胞的活动，影响骨代谢平衡，还可参与能量代谢、糖脂代谢等，有望成为骨代谢疾病等领域的治疗靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于能量代谢研究、骨发育调控机制探索、运动生理学实验等科研领域。可广泛应用于肥胖模型、骨质疏松症研究或生物节律相关实验中的OSTN动态监测，为体外培养细胞或动物模型中的OSTN表达分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ostn ELISA Kit; Osteocrin ELISA Kit; Musclin) [Cleaved into: Processed Osteocrin] ELISA Kit
缩写：OSTN
Uniprot No.：P61364
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse OSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 92 | ||||
| Range % | 87-97 | |||||
| 1:10 | Average % | 95 | ||||
| Range % | 88-99 | |||||
| 1:20 | Average % | 93 | ||||
| Range % | 87-100 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 87-104 | |||||
回收率：
| The recovery of mouse OSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 85-99 | ||||
| EDTA plasma (n=4) | 97 | 89-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.5792.6052.5922.5025001.4471.4941.4711.3812500.7910.8030.7970.7071250.4300.4270.4290.33962.50.2190.2250.2220.13231.20.1440.1490.1470.05715.60.1070.1120.1100.02000.0880.0910.090 | ||||||
货期：3-5 working days
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