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产品描述：人鸟氨酸氨甲酰基转移酶,线粒体(OTC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的OTC含量。试剂盒检测范围为1.25 mU/mL-80 mU/mL，适用于科研领域中对肝代谢疾病模型、遗传性尿素循环障碍机制、药物肝毒性评估以及细胞能量代谢相关研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EC 2.1.3.3 ELISA Kit; MGC129967 ELISA Kit; MGC129968 ELISA Kit; MGC138856 ELISA Kit; OCTD ELISA Kit; Ornithine Carbamoyltransferase ELISA Kit; Ornithine carbamoyltransferase mitochondrial ELISA Kit; Ornithine carbamoyltransferase, mitochondrial ELISA Kit; Ornithine transcarbamylase ELISA Kit; Otc ELISA Kit; OTC_HUMAN ELISA Kit; OTCase ELISA Kit
缩写：OTC
Uniprot No.：P00480
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：1.25 mU/mL-80 mU/mL
灵敏度：0.312 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human OTC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 84 | |||||
| Range % | 80-89 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 94-103 | ||||||
| 1:4 | Average % | 85 | |||||
| Range % | 81-89 | ||||||
| 1:8 | Average % | 99 | |||||
| Range % | 94-104 | ||||||
回收率：
| The recovery of human OTC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 100 | 95-105 | |||||
| EDTA plasma (n=4) | 86 | 83-89 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| mU/mlOD1OD2AverageCorrected801.9641.9001.9321.748401.7641.7891.7771.593201.5791.5021.5411.357101.2571.2011.2291.04550.9010.9310.9160.7322.50.5910.5870.5890.4051.250.3620.3710.3670.18300.1880.1800.184 | |||||||
货期：3-5 working days
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