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产品描述：猪催产素(OT)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的OT含量。试剂盒检测范围为2.5 pg/mL-100 pg/mL，科研人员可通过探究母猪分娩机制、泌乳调控规律，或分析OT在社交行为及环境适应中的分子调控网络，尤其适用于比较医学模型构建及畜牧养殖相关激素代谢研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MGC126890, MGC126892, OT, OT-NPI, neurophysin I|oxytocin, prepro- (neurophysin I)|oxytocin-neurophysin I|oxytocin-neurophysin I, preproprotein
缩写：OT
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：2.5 pg/mL-100 pg/mL
灵敏度：2.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig oxytocin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-99 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-101 | |||||
回收率：
| The recovery of pig oxytocin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-96 | ||||
| EDTA plasma (n=4) | 96 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 02.4512.4282.440 2.51.0521.0291.041 7.50.5510.5360.544 230.3810.3920.387 500.2750.2620.269 1000.1440.1420.143 | ||||||
货期：3-5 working days
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