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产品描述：人Repetin蛋白(RPTN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的RPTN含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，该产品适用于科研领域中对皮肤生理病理机制、角质形成细胞功能研究，以及化妆品或药物对表皮屏障影响的体外评估等场景，可为皮肤生物学、疾病模型构建及相关转化研究提供可靠的工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：RPTN ELISA Kit; Repetin ELISA Kit
缩写：RPTN
Uniprot No.：Q6XPR3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human RPTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 88-97 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 87-102 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 89-97 | |||||
回收率：
| The recovery of human RPTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-98 | ||||
| EDTA plasma (n=4) | 90 | 87-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.7962.8692.8332.711202.1311.9942.0631.941101.1521.2431.1981.07650.7050.7320.7190.5972.50.3430.3660.3550.2331.250.2360.2240.2300.1080.6250.2180.2100.2140.09200.1240.1200.122 | ||||||
货期：3-5 working days
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