首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人S100蛋白(S-100)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的S100A1含量。S100A1属于S100蛋白家族成员，在多种组织中表达。其参与细胞内钙离子信号传导、能量代谢等多种生理过程。研究发现它与心肌收缩、肿瘤进展等机制相关，有望成为心血管疾病和癌症等治疗的潜在靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于神经生物学、肿瘤微环境研究、炎症机制探索等基础科研场景，尤其可为脑损伤模型评估、胶质瘤研究或免疫调控实验提供可靠的生物标志物数据支持，助力研究者深入解析S100蛋白在疾病发生发展中的分子作用机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Bpb ELISA Kit; NEF ELISA Kit; Protein S100-A1 ELISA Kit; S-100 protein alpha chain ELISA Kit; S-100 protein subunit alpha ELISA Kit; S100 alpha ELISA Kit; S100 beta ELISA Kit; S100 calcium binding protein A1 ELISA Kit; S100 calcium binding protein B ELISA Kit; S100 calcium-binding protein A1 ELISA Kit; S100 protein alpha polypeptide ELISA Kit; S100A ELISA Kit; s100a1 ELISA Kit; S100B ELISA Kit; S100beta ELISA Kit; S10A1_HUMAN ELISA Kit
缩写：S100A1
Uniprot No.：P23297
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human S-100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 82-90 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 97-103 | |||||
回收率：
| The recovery of human S-100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.4012.4382.4202.28451.8921.8711.8821.7462.51.2121.2341.2231.0871.250.6610.6980.6800.5440.6250.3640.3840.3740.2380.3120.2830.2610.2720.1360.1560.2060.2130.2100.07400.1350.1370.136 | ||||||
货期：3-5 working days
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