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产品描述：人氨酰tRNA合成酶复合多功能相互作用蛋白1(SCYE1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SCYE1含量。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，在动脉粥样硬化、急性期反应等病理机制研究中具有重要价值；可为炎症相关信号通路研究、疾病模型分子机制探索、药物干预效果评价等基础科研提供可靠数据支持，宽泛的样本兼容性尤其适合多维度实验设计，例如同时检测生物体液和细胞组织中的蛋白表达水平变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AIMP 1 ELISA Kit; Aimp1 ELISA Kit; AIMP1/p43 ELISA Kit; AIMP1_HUMAN ELISA Kit; Aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 ELISA Kit; ARS interacting multifunctional protein 1 ELISA Kit; EMAP 2 ELISA Kit; EMAP-2 ELISA Kit; EMAP-II ELISA Kit; EMAP2 ELISA Kit; EMAPII ELISA Kit; Endothelial monocyte activating polypeptide 2 ELISA Kit; Endothelial monocyte activating polypeptide ELISA Kit; Endothelial monocyte-activating polypeptide 2 ELISA Kit; Endothelial monocyte-activating polypeptide II ELISA Kit; HLD3 ELISA Kit; Multisynthase complex auxiliary component p43 ELISA Kit; Multisynthetase complex auxiliary component p43 ELISA Kit; OTTHUMP00000219673 ELISA Kit; p43 ELISA Kit; Scye 1 ELISA Kit; Scye1 ELISA Kit; Scye1, formerly ELISA Kit; Small inducible cytokine subfamily E member 1 ELISA Kit; Small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) ELISA Kit; Small inducible cytokine subfamily E, member 1, formerly ELISA Kit
缩写：SCYE1
Uniprot No.：Q12904
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SCYE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 102 | |||||
| Range % | 95-106 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 96-105 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 86-94 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 89-98 | ||||||
回收率：
| The recovery of human SCYE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 104 | 98-108 | |||||
| EDTA plasma (n=4) | 93 | 86-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected15001.9761.9331.9551.8007501.3651.2841.3251.1703750.8720.8280.8500.695187.50.5780.5450.5620.407940.3880.3610.3750.220470.2510.2460.2490.09423.50.2190.2150.2170.06200.1560.1540.155 | |||||||
货期：3-5 working days
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