首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠信号肽、CUB和EGF样结构域蛋白1(SCUBE1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SCUBE1含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本试剂盒适用于基础科研领域，如探究SCUBE1在疾病模型中的表达调控机制、评估基因敲除或药物干预后的蛋白水平变化，以及作为相关信号通路研究的生物标志物检测工具，满足体外实验中对SCUBE1蛋白定量分析的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Scube1 ELISA Kit; Signal peptide ELISA Kit; CUB and EGF-like domain-containing protein 1 ELISA Kit
缩写：SCUBE1
Uniprot No.：Q6NZL8
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SCUBE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:10 | Average % | 101 | ||||
| Range % | 96-104 | |||||
| 1:20 | Average % | 92 | ||||
| Range % | 87-95 | |||||
| 1:40 | Average % | 98 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of mouse SCUBE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
| EDTA plasma (n=4) | 107 | 99-111 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.8712.7752.8232.71152.0541.9892.0221.9102.51.1231.1381.1311.0191.250.6450.6750.6600.5480.6250.3720.3840.3780.2660.3120.2870.2770.2820.1700.1560.1960.1890.1930.08100.1130.1110.112 | ||||||
货期：3-5 working days
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