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产品描述：人信号肽,CUB和EGF样结构域蛋白1(SCUBE1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SCUBE1含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于基础科研中探索SCUBE1在疾病模型中的表达动态、信号通路关联性分析，或作为分子标志物在细胞实验、动物模型及临床前样本中的定量评估，为研究血管生成、组织修复及病理机制提供可靠工具。满足分子生物学及转化医学研究的精准检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SCUB1_HUMAN ELISA Kit; SCUBE 1 ELISA Kit; SCUBE1 ELISA Kit; Signal peptide, CUB and EGF-like domain-containing protein 1 ELISA Kit; Signal peptide, CUB domain, EGF like 1 ELISA Kit
缩写：SCUBE1
Uniprot No.：Q8IWY4
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SCUBE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 89 | ||||
| Range % | 82-94 | |||||
| 1:10 | Average % | 96 | ||||
| Range % | 91-105 | |||||
| 1:20 | Average % | 99 | ||||
| Range % | 95-108 | |||||
| 1:40 | Average % | 94 | ||||
| Range % | 86-102 | |||||
回收率：
| The recovery of human SCUBE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 106 | 99-110 | ||||
| EDTA plasma (n=4) | 92 | 84-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.6702.5922.6312.502202.2712.1732.2222.093101.6311.6151.6231.49450.9400.9250.9330.8042.50.5120.5020.5070.3781.250.3570.3470.3520.2230.6250.2260.2190.2230.09400.1300.1270.129 | ||||||
货期：3-5 working days
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