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产品描述：人Syndecan-1/CD138(SDC1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SDC1含量。SDC1（Syndecan-1）是硫酸肝素蛋白多糖家族的重要成员，主要存在于上皮细胞，参与细胞粘附、信号转导和肿瘤发生发展。研究显示，SDC1通过调节细胞增殖、凋亡和迁移等过程，在多种肿瘤中发挥重要作用，是乳腺癌等肿瘤的潜在治疗靶点。试剂盒检测范围为4.7 ng/mL-300 ng/mL，特别适用于肿瘤生物学、免疫调节机制或组织再生等基础研究领域，为研究者探索SDC1在疾病发生发展中的分子作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD_antigen ELISA Kit; CD138 ELISA Kit; CD138 antigen ELISA Kit; heparan sulfate proteoglycan fibroblast growth factor receptor ELISA Kit; SDC ELISA Kit; Sdc1 ELISA Kit; SDC1_HUMAN ELISA Kit; SYND1 ELISA Kit; Syndecan 1 ELISA Kit; Syndecan ELISA Kit; syndecan proteoglycan 1 ELISA Kit; Syndecan-1 ELISA Kit
缩写：SDC1
Uniprot No.：P18827
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：4.7 ng/mL-300 ng/mL
灵敏度：1.17 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SDC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 82-91 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-101 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 88-99 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human SDC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 93-99 | ||||
| EDTA plasma (n=4) | 90 | 84-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected3002.4392.4112.4252.2721502.0482.0012.0251.872751.6351.5471.5911.43837.51.1821.1061.1440.99118.750.8430.7810.8120.6599.40.4670.4380.4530.3004.70.2820.2740.2780.12500.1590.1470.153 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human SDC1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled SDC1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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