首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠多配体聚糖1(SDC1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SDC1含量。SDC1（Syndecan-1）是一种硫酸肝素蛋白聚糖，主要在上皮细胞和浆细胞中表达。研究发现，SDC1在多种肿瘤中表达上调，与不良预后有关。SDC1通过其胞外段硫酸肝素链与多种配体结合，参与细胞信号转导，在肿瘤细胞的增殖、凋亡、血管生成、迁移侵袭和耐药等过程中发挥重要作用。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于多种科研场景，包括探索肿瘤生长、炎症性疾病或组织修复过程中SDC1的表达动态，分析其在细胞迁移、增殖中的功能机制，以及评估不同器官（如肝、肾）病理模型中SDC1的生物学作用。为研究SDC1在疾病模型中的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sdc1; Synd-1; Synd1; Syndecan-1; SYND1; CD antigen CD138
缩写：SDC1
Uniprot No.：P18828
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SDC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-97 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-99 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of mouse SDC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-95 | ||||
| EDTA plasma (n=4) | 95 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.8442.8722.8582.77120001.9872.0071.9971.91010001.1321.1381.1351.0485000.6310.6200.6260.5392500.3640.3550.3600.2731250.2410.2360.2390.15262.50.1580.1520.1550.06800.0880.0860.087 | ||||||
货期：3-5 working days
×
