| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSR2 |
| Uniprot No | Q969E8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-190aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAGAAEDARALFRAGVCAALEAWPALQ IAVENGFGGVHSQEKAKWLGGAVEDYFMRNADLELDEVEDFLGELLTNEF DTVVEDGSLPQVSQQLQTMFHHFQRGDGAALREMASCITQRKCKVTATAL KTARETDEDDDVDSVEEMEVTATNDGAATDGVCPQPEPSDPDAQTIKEED IVEDGWTIVRRK |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TSR2重组蛋白的假想参考文献示例，基于领域内常见研究方向构建：
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1. **文献名称**：*Structural and functional characterization of the TSR2 protein in ribosome biogenesis*
**作者**：Smith A, et al.
**摘要**：本研究通过大肠杆菌表达系统纯化重组人TSR2蛋白，结合X射线晶体学解析其三维结构，发现TSR2通过特定结构域与RNA结合，并验证其在核糖体RNA加工中的关键作用，为理解核糖体合成异常相关疾病提供依据。
2. **文献名称**：*TSR2 mutations disrupt cell cycle regulation in acute lymphoblastic leukemia*
**作者**：Chen L, et al.
**摘要**：文章报道TSR2基因在白血病患者中的高频突变，利用重组TSR2蛋白进行体外功能实验，发现突变体导致细胞周期蛋白依赖性激酶(CDK)活性异常，阐明其通过干扰RNA代谢影响细胞周期进程的机制。
3. **文献名称**：*Development of a high-yield mammalian expression system for recombinant TSR2 production*
**作者**：Wang Y, et al.
**摘要**：研究优化了哺乳动物细胞中TSR2重组蛋白的表达条件，实现高纯度制备，并证明其与天然蛋白功能一致性，为基于TSR2的药物筛选和抗体开发提供了可靠工具。
4. **文献名称**：*TSR2 interacts with MYC oncoprotein: Implications for cancer therapeutics*
**作者**：Johnson R, et al.
**摘要**：通过重组TSR2蛋白的pull-down实验，发现其与MYC蛋白直接互作，调控靶基因转录。研究提示靶向TSR2-MYC复合物可能成为抑制肿瘤生长的潜在策略。
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注：以上文献为示例性内容，实际研究中请通过学术数据库检索真实发表论文。
**Background of TSR2 Recombinant Protein**
TSR2 (Thrombospondin Type-1 Repeat-containing protein 2) is a protein encoded by the *TSR2* gene, which plays roles in diverse biological processes, including cell adhesion, angiogenesis, and extracellular matrix (ECM) interactions. The protein is characterized by the presence of thrombospondin type-1 repeats (TSRs), conserved domains known to mediate interactions with other ECM components, cell surface receptors (e.g., CD36. integrins), and growth factors. These interactions enable TSR2 to regulate cellular signaling pathways involved in tissue development, wound healing, and immune responses.
Recombinant TSR2 protein is engineered using biotechnological methods, often expressed in systems like *E. coli* or mammalian cells, followed by purification to ensure high specificity and activity. Its production allows researchers to study TSR2’s structure-function relationships, ligand-binding properties, and roles in pathologies such as cancer (e.g., modulating tumor angiogenesis) or inflammatory diseases.
Studies highlight TSR2’s dual role as both a promoter and inhibitor of angiogenesis, depending on cellular context, making it a potential therapeutic target. Recombinant TSR2 is also utilized in drug discovery, serving as a tool to screen molecules that modulate its interactions with receptors like CD36 or TGF-β. Additionally, its involvement in microbial pathogenesis (e.g., *Plasmodium falciparum* infection mechanisms) underscores its relevance in infectious disease research.
Overall, TSR2 recombinant protein serves as a critical reagent for unraveling ECM biology, disease mechanisms, and therapeutic development.
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