首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：猪分泌型免疫球蛋白A(sIgA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的sIgA含量。sIgA即分泌型免疫球蛋白A，是黏膜免疫的主要效应分子。其能在黏膜表面阻止病原体黏附与入侵，中和毒素等。研究中聚焦于其产生机制、影响因素等，以探索增强sIgA水平或功能的方法，用于防治黏膜感染等疾病。试剂盒检测范围为0.15 μg/mL-37.5 μg/mL，适用于动物免疫机制研究，如评估肠道感染模型中的黏膜免疫应答、疫苗效价验证或饲料添加剂对免疫调节的影响等领域。支持科研人员在体外实验中系统性分析sIgA的动态变化，为猪类免疫学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：sIgA
种属：Sus scrofa (Pig)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.15 μg/mL-37.5 μg/mL
灵敏度：0.15 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:400 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:800 | Average % | 87 | ||||
| Range % | 85-94 | |||||
| 1:1600 | Average % | 96 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of pig sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 103 | 98-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 37.50.1200.1150.118 9.380.4560.4630.460 2.340.9010.8770.889 0.581.4321.5271.480 0.152.1032.1892.146 02.5442.7532.649 | ||||||
货期：3-5 working days
×
