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产品描述:猪多肽YY(PYY)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆样本中的PYY含量。PYY即肽YY,是由肠道L细胞分泌的一种胃肠激素。它能减少胃肠道蠕动、抑制胃酸分泌等。其研究机制主要围绕与受体结合发挥作用,在调节食欲、能量代谢等方面有重要意义,为肥胖、糖尿病等疾病的治疗提供潜在靶点。试剂盒检测范围为62.5 pg/mL-1000 pg/mL,该产品适用于科研领域探索猪的消化生理机制、营养干预研究或代谢相关疾病模型的构建,例如肥胖、糖尿病等动物实验中的激素动态监测,为解析 PYY 在能量稳态中的作用提供可靠工具。适用于基础医学、兽医学及动物营养学等领域的科学研究。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:PYY ELISA Kit; Peptide YY ELISA Kit; PYY ELISA Kit; Peptide tyrosine tyrosine) [Cleaved into: Peptide YY(3-36 ELISA Kit; PYY-II)] ELISA Kit
种属:Sus scrofa (Pig)
样本类型:serum, plasma
检测范围:62.5 pg/mL-1000 pg/mL
灵敏度:16.88 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Neuroscience
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of pig PYY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 92 | ||||
Range % | 87-99 | |||||
1:2 | Average % | 96 | ||||
Range % | 91-100 | |||||
1:4 | Average % | 94 | ||||
Range % | 89-98 | |||||
1:8 | Average % | 93 | ||||
Range % | 87-99 |
回收率:
The recovery of pig PYY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 96 | 91-101 | ||||
EDTA plasma (n=4) | 94 | 89-99 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pg/mlOD1OD2Average 10000.1710.1750.173 5000.3520.3530.353 2500.6810.6760.679 1251.2691.2721.271 62.52.8582.8672.863 |
货期:3-5 working days
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