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产品描述：猪缪勒管抑制物质/抗缪勒管激素(AMH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AMH含量。抗缪勒管激素（AMH）是转化生长因子β超家族成员。在女性卵泡生长发育中起重要调控作用，可反映卵巢储备功能。研究机制上，其参与调节卵泡募集和生长，且水平与窦前卵泡和小窦卵泡数量相关，是评估卵巢功能的关键指标。试剂盒检测范围为1.25 ng/mL-50 ng/mL，适用于动物生殖生理研究、繁殖效率评估、卵巢功能分析等科研领域，特别为猪科动物生殖内分泌研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AMH ELISA Kit; Muellerian-inhibiting factor ELISA Kit; Anti-Muellerian hormone ELISA Kit; AMH ELISA Kit; Muellerian-inhibiting substance ELISA Kit; MIS ELISA Kit
缩写：AMH
Uniprot No.：P79295
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-50 ng/mL
灵敏度：1.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig AMH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 89-102 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 90-103 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-100 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 83-107 | |||||
回收率：
| The recovery of pig AMH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-103 | ||||
| EDTA plasma (n=4) | 97 | 88-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 500.1340.1390.137 250.2270.2380.233 11.50.3300.3430.337 3.750.6110.5920.602 1.251.2641.2141.239 02.6862.6352.661 | ||||||
货期：3-5 working days
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