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产品描述：猪生长激素(GH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GH1含量。GH1 基因编码生长激素，生长激素在人体生长发育、代谢调节等方面至关重要。其相关研究机制围绕生长激素的分泌调节、信号通路、功能作用等展开。如对其分泌调控机制的研究，有助于了解生长发育异常疾病，为临床治疗提供思路。试剂盒检测范围为2.5 ng/mL-100 ng/mL，适用于动物生理学、营养代谢研究及生长调控机制探索等科研领域。可为畜牧养殖优化、饲料效能评估等研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
缩写：GH1
Uniprot No.：P01248
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：2.5 ng/mL-100 ng/mL
灵敏度：2.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 88-99 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-103 | |||||
回收率：
| The recovery of pig GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-102 | ||||
| EDTA plasma (n=4) | 98 | 92-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 01.9051.8341.870 2.51.2981.2821.290 7.51.0241.0041.014 230.7390.7220.731 500.5670.5310.549 1000.3420.3380.340 | ||||||
货期：3-5 working days
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