首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠心肌肌钙蛋白(cTn-Ⅰ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的TNNI3含量。TNNI3是一个重要靶点。它是心肌肌钙蛋白I的编码基因，在心肌收缩调控中起关键作用。研究围绕其结构与功能，探索突变对心肌功能影响及疾病关联机制，有望为心肌病等心脏疾病的诊断、治疗提供新方向和靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于多种科研场景，包括心血管疾病机制研究、心肌损伤动物模型验证、心脏保护药物疗效评估以及环境毒理或药理学实验中心肌损伤程度的动态监测。为研究小鼠心脏病理生理变化、筛选心肌保护化合物提供可靠工具，广泛应用于基础医学、药理学及毒理学等领域的科研探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tnni3 ELISA Kit; Troponin I ELISA Kit; cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit
缩写：TNNI3
Uniprot No.：P48787
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 88-95 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 97-105 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 82-88 | |||||
回收率：
| The recovery of mouse cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-95 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.3992.3562.3782.221 5001.6101.6871.6491.492 2500.9550.9970.9760.819 1250.5630.5680.5660.409 62.50.3750.3580.3670.210 31.20.2410.2360.2390.082 15.60.1650.1780.1720.015 00.1540.1590.157 | ||||||
货期：3-5 working days
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