| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF10 |
| Uniprot No | P21506 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-573aa |
| 氨基酸序列 | MDAKSLTAWS RTLVTFKDVF VDFTREEWKL LDTAQQIVYR NVMLENYKNL VSLGYQLTKP DVILRLEKGE EPWLVEREIH QETHPDSETA FEIKSSVSSR SIFKDKQSCD IKMEGMARND LWYLSLEEVW KCRDQLDKYQ ENPERHLRQV AFTQKKVLTQ ERVSESGKYG GNCLLPAQLV LREYFHKRDS HTKSLKHDLV LNGHQDSCAS NSNECGQTFC QNIHLIQFAR THTGDKSYKC PDNDNSLTHG SSLGISKGIH REKPYECKEC GKFFSWRSNL TRHQLIHTGE KPYECKECGK SFSRSSHLIG HQKTHTGEEP YECKECGKSF SWFSHLVTHQ RTHTGDKLYT CNQCGKSFVH SSRLIRHQRT HTGEKPYECP ECGKSFRQST HLILHQRTHV RVRPYECNEC GKSYSQRSHL VVHHRIHTGL KPFECKDCGK CFSRSSHLYS HQRTHTGEKP YECHDCGKSF SQSSALIVHQ RIHTGEKPYE CCQCGKAFIR KNDLIKHQRI HVGEETYKCN QCGIIFSQNS PFIVHQIAHT GEQFLTCNQC GTALVNTSNL IGYQTNHIRE NAY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZNF10重组蛋白的假设性参考文献(文献信息为示例，非真实存在)：
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1. **文献名称**: *Cloning and Functional Analysis of Recombinant ZNF10 in DNA Binding*
**作者**: Smith A, et al.
**摘要**: 本研究成功克隆了人源ZNF10基因，并在大肠杆菌系统中表达纯化了重组ZNF10蛋白。通过电泳迁移率实验(EMSA)证实其特异性结合靶DNA序列，揭示了其在转录调控中的潜在作用。
2. **文献名称**: *Expression and Subcellular Localization of FLAG-Tagged ZNF10 in Mammalian Cells*
**作者**: Johnson R, Lee S.
**摘要**: 构建了FLAG标签的ZNF10重组蛋白表达载体，转染HEK293细胞后通过免疫荧光技术观察到ZNF10定位于细胞核。Western blot和荧光素酶报告基因实验表明其通过KRAB结构域抑制靶基因转录。
3. **文献名称**: *Crystal Structure of ZNF10 Recombinant Protein Reveals Zinc Finger Domain Architecture*
**作者**: Zhang Y, et al.
**摘要**: 利用昆虫细胞表达系统制备了高纯度ZNF10重组蛋白，并通过X射线晶体学解析其三维结构，明确了锌指基序的空间排列及与DNA相互作用的分子机制。
4. **文献名称**: *ZNF10 Interaction with Histone Deacetylases: A Role in Epigenetic Silencing*
**作者**: Müller C, et al.
**摘要**: 通过体外Pull-down实验和质谱分析，发现重组ZNF10蛋白与HDAC复合物直接相互作用，提示其可能通过招募组蛋白修饰酶参与染色质重塑和基因沉默。
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**说明**：以上文献为基于领域知识的模拟示例，实际研究中请通过PubMed、Web of Science等平台检索真实文献。
**Background of ZNF10 Recombinant Protein**
ZNF10 (Zinc Finger Protein 10) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (ZFP) family, which represents one of the largest groups of transcription regulators in mammals. These proteins are characterized by tandem C2H2-type zinc finger motifs that mediate sequence-specific DNA binding, coupled with a KRAB domain at the N-terminus that often recruits co-repressors to regulate gene expression epigenetically. ZNF10. specifically, is implicated in transcriptional repression, potentially influencing cellular processes such as differentiation, proliferation, and apoptosis.
The recombinant ZNF10 protein is engineered for experimental studies to elucidate its structure, DNA-binding specificity, and functional interactions. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant form typically retains the zinc finger domains and KRAB region, enabling *in vitro* analyses like electrophoretic mobility shift assays (EMSAs) or chromatin immunoprecipitation (ChIP). Its production often involves affinity tags (e.g., GST, His-tag) for purification and detection.
Research on ZNF10 has explored its role in development, cancer, and neurological disorders, with studies suggesting it may suppress oncogenic pathways or regulate genes involved in neural plasticity. The recombinant protein serves as a critical tool for dissecting mechanisms of KRAB-ZFP-mediated gene silencing and its broader implications in health and disease.
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