纯度 | >85%SDS-PAGE. |
种属 | Human |
靶点 | ZMYND19 |
Uniprot No | Q96E35 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-227aa |
氨基酸序列 | MTDFKLGIVR LGRVAGKTKY TLIDEQDIPL VESYSFEARM EVDADGNGAK IFAYAFDKNR GRGSGRLLHE LLWERHRGGV APGFQVVHLN AVTVDNRLDN LQLVPWGWRP KAEETSSKQR EQSLYWLAIQ QLPTDPIEEQ FPVLNVTRYY NANGDVVEEE ENSCTYYECH YPPCTVIEKQ LREFNICGRC QVARYCGSQC QQKDWPAHKK HCRERKRPFQ HELEPER |
预测分子量 | kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZMYND19重组蛋白的假设性参考文献示例(注:ZMYND19相关研究较少,以下内容为模拟虚构,建议通过PubMed或Google Scholar查询真实文献):
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1. **Title**: *ZMYND19 regulates mitochondrial dynamics through interaction with fusion proteins*
**Author**: Li X, et al.
**Summary**: 本研究利用重组ZMYND19蛋白进行体外结合实验,发现其与线粒体融合蛋白MFN2存在直接相互作用,并通过敲低实验证明ZMYND19缺失导致线粒体碎片化,提示其在维持线粒体网络稳态中的作用。
2. **Title**: *Crystal structure of the ZMYND19 N-terminal domain reveals a novel chromatin-binding motif*
**Author**: Wang Y, et al.
**Summary**: 通过重组表达并解析ZMYND19氨基端结构域的晶体结构,发现其含有一个新型的α螺旋-转角-α螺旋模体,可能参与染色质重塑复合物的招募,为研究其在表观遗传调控中的功能提供结构基础。
3. **Title**: *ZMYND19 acts as a tumor suppressor in colorectal cancer by modulating HIF-1α signaling*
**Author**: Chen H, et al.
**Summary**: 利用重组ZMYND19蛋白进行细胞回补实验,发现其通过泛素化降解HIF-1α抑制结直肠癌细胞侵袭,临床数据分析显示ZMYND19低表达与患者不良预后相关。
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**注意**:以上文献为示例性质,实际研究中请通过学术数据库检索关键词“ZMYND19 recombinant protein”或结合具体研究领域(如癌症、细胞器调控等)筛选文献。
ZMYND19 (Zinc Finger MYND Domain-Containing Protein 19) is a relatively less-characterized protein encoded by the *ZMYND19* gene in humans. It belongs to the MYND domain-containing protein family, which typically functions as adaptors in chromatin remodeling and transcriptional regulation. The MYND domain, a conserved zinc finger motif, mediates protein-protein interactions, particularly with co-repressor complexes or histone-modifying enzymes. ZMYND19 is proposed to play roles in transcriptional regulation, epigenetic modulation, and cellular processes such as DNA repair or ciliogenesis. However, its precise molecular mechanisms and physiological significance remain under investigation.
Recombinant ZMYND19 protein refers to the purified, engineered form of the protein produced via heterologous expression systems, such as *E. coli* or mammalian cell cultures. This allows controlled study of its biochemical properties, interactions, and functions *in vitro*. Researchers often clone the *ZMYND19* cDNA into expression vectors with affinity tags (e.g., His-tag, GST-tag) to facilitate purification. Structural studies using recombinant protein aim to map functional domains, such as its N-terminal PHD finger or C-terminal MYND domain, which may bind methylated histones or recruit chromatin modifiers like HDACs.
Interest in ZMYND19 stems from its potential links to diseases. For example, mutations in *ZMYND19* have been associated with mitochondrial complex III deficiency, suggesting a role in mitochondrial metabolism. Its interaction partners and expression patterns in tissues like the brain and testes hint at tissue-specific regulatory roles. Recombinant ZMYND19 enables functional assays (e.g., pull-downs, enzymatic activity screens) to dissect these pathways. Further research may clarify its involvement in developmental disorders, cancer, or ciliopathies, positioning it as a target for therapeutic exploration.
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