关键信息
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基因名
GSK3a
- 应用
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别名
GSK3a；Glycogen synthase kinase-3 alpha
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P49840
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表达区间
2-483aa
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氨基酸序列
SGGGPSGGG PGGSGRARTS SFAEPGGGGG GGGGGPGGSA SGPGGTGGGK ASVGAMGGGV GASSSGGGPG GSGGGGSGGP GAGTSFPPPG VKLGRDSGKV TTVVATLGQG PERSQEVAYT DIKVIGNGSF GVVYQARLAE TRELVAIKKV LQDKRFKNRE LQIMRKLDHC NIVRLRYFFY SSGEKKDELY LNLVLEYVPE TVYRVARHFT KAKLTIPILY VKVYMYQLFR SLAYIHSQGV CHRDIKPQNL LVDPDTAVLK LCDFGSAKQL VRGEPNVSYI CSRYYRAPEL IFGATDYTSS IDVWSAGCVL AELLLGQPIF PGDSGVDQLV EIIKVLGTPT REQIREMNPN YTEFKFPQIK AHPWTKVFKS RTPPEAIALC SSLLEYTPSS RLSPLEACAH SFFDELRCLG TQLPNNRPLP PLFNFSAGEL SIQPSLNAIL IPPHLRSPAG TTTLTPSSQA LTETPTSSDW QSTDATPTLT NSS
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分子量
51 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
GSK3a, or Glycogen Synthase Kinase 3 alpha, is a serine/threonine kinase that plays a critical role in various cellular processes, including glycogen metabolism, cell signaling, and the regulation of gene expression. It is highly involved in the Wnt signaling pathway, which is essential for embryonic development and stem cell differentiation, and aberrations in GSK3a function have been implicated in numerous diseases, including cancer, Alzheimer's disease, and diabetes. The study of GSK3a recombinant protein has gained significant attention due to its potential as a therapeutic target; understanding its structure and function can provide insights into its regulatory mechanisms. By investigating GSK3a in detail, researchers aim to elucidate its role in disease pathology and develop targeted treatments that modulate its activity. The recombinant production of GSK3a allows for the generation of pure protein samples, which can be used in biochemical assays, structural studies, and drug discovery efforts. Moreover, studying the enzymatic properties and interactions of GSK3a with various substrates and inhibitors can help identify novel therapeutic compounds to combat diseases associated with its dysregulation. As such, GSK3a recombinant protein research holds promise in advancing our understanding of cellular signaling pathways and developing innovative strategies for disease intervention.
