| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | CNTN3 |
| Uniprot No | Q07409 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1001aa |
| 氨基酸序列 | MMLSWKQLIL LSFIGCLAGE LLLQGPVFIK EPSNSIFPVD SEDKKITLNC EARGNPSPHY RWQLNGSDID TSLDHRYKLN GGNLIVINPN RNWDTGSYQC FATNSLGTIV SREAKLQFAY LENFKTRMRS TVSVREGQGV VLLCGPPPHS GELSYAWVFN EYPSFVEEDS RRFVSQETGH LYIAKVEPSD VGNYTCVVTS TVTNTRVLGS PTPLVLRSDG VMGEYEPKIE VQFPETLPAA KGSTVRLECF ALGNPVPQIN WRRSDGMPFP NKIKLRKFNG MLEIQNFQQE DTGSYECIAE NSRGKNVARG RLTYYAKPYW LQLLRDVEIA VEDSLYWECR ASGKPKPSYR WLKNGDALVL EERIQIENGA LTITNLNVTD SGMFQCIAEN KHGLIYSSAE LKVVASAPDF SRNPMKKMVQ VQVGSLVILD CKPRASPRAL SFWKKGDMMV REQARVSFLN DGGLKIMNVT KADAGTYTCT AENQFGKANG TTHLVVTEPT RIILAPSNMD VAVGESVILP CQVQHDPLLD IMFAWYFNGA LTDFKKDGSH FEKVGGSSSG DLMIRNIQLK HSGKYVCMVQ TGVDSVSSAA ELIVRGSPGP PENVKVDEIT DTTAQLSWTE GTDSHSPVIS YAVQARTPFS VGWQSVRTVP EVIDGKTHTA TVVELNPWVE YEFRIVASNK IGGGEPSLPS EKVRTEEAAP EIAPSEVSGG GGSRSELVIT WDPVPEELQN GGGFGYVVAF RPLGVTTWIQ TVVTSPDNPR YVFRNESIVP FSPYEVKVGV YNNKGEGPFS PVTTVFSAEE EPTVAPSHIS AHSLSSSEIE VSWNTIPWKL SNGHLLGYEV RYWNNGGEEE SSRKVKVAGN QTSAVLRGLK SNLAYYTAVR AYNSAGAGPF SATVNATTKK TPPSQPPGNV VWNATDTKVL LNWEQVKAME NESEVTGYKV FYRTSSQNNV HVLNTNKTSA ELLLPIKEDY IIEVKATTDG GDGTSSEQIR IPRITSMDAR G |
| 预测分子量 | 110 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CNTN3重组蛋白的相关文献示例(注：文献信息为模拟生成，仅供参考)：
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1. **文献名称**：Structural and functional analysis of recombinant CNTN3 in neural adhesion
**作者**：Li X, et al.
**摘要**：研究通过昆虫细胞系统表达CNTN3重组蛋白，解析其Ig结构域的三维构象，并验证其在神经元轴突导向中的黏附功能。
2. **文献名称**：CNTN3 recombinant protein promotes oligodendrocyte differentiation via F3/contactin signaling
**作者**：Wang Y, et al.
**摘要**：利用哺乳动物细胞表达系统获得高纯度CNTN3重组蛋白，发现其通过与F3受体相互作用激活下游ERK通路，促进少突胶质细胞分化。
3. **文献名称**：Autoantibody detection using recombinant CNTN3 in autoimmune neuropathy
**作者**：Tanaka K, et al.
**摘要**：通过大肠杆菌表达CNTN3重组蛋白片段，开发ELISA检测方法，发现其在慢性炎症性脱髓鞘性神经病变患者血清中存在特异性自身抗体。
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**说明**：以上文献为示例性质，实际研究需通过PubMed、Google Scholar等平台检索关键词(如"CNTN3 recombinant protein")。建议结合具体研究方向补充筛选。
**Background of CNTN3 Recombinant Protein**
CNTN3 (Contactin 3), a member of the contactin protein family, is a cell adhesion molecule (CAM) predominantly expressed in the nervous system. It plays a critical role in neural development, synaptic plasticity, and axon guidance by mediating cell-cell interactions through homophilic or heterophilic binding. Structurally, CNTN3 contains six immunoglobulin-like domains and four fibronectin type III repeats, characteristic of proteins involved in intercellular signaling and neuronal network formation. Dysregulation of CNTN3 has been implicated in neurological disorders, including autism spectrum disorders, schizophrenia, and Alzheimer’s disease, highlighting its importance in maintaining neural integrity.
Recombinant CNTN3 protein is engineered using mammalian or bacterial expression systems to ensure proper folding and post-translational modifications. This allows researchers to study its biochemical properties, ligand-receptor interactions, and functional roles in vitro or in animal models. The purified protein is commonly utilized in assays investigating neurite outgrowth, cell migration, and synaptic connectivity. Additionally, it serves as a tool for developing therapeutic antibodies or small molecules targeting CNTN3-associated pathways.
Recent studies also explore CNTN3’s potential role in cancer, as aberrant expression has been observed in certain tumors, suggesting involvement in metastasis or immune evasion. Its recombinant form enables mechanistic studies to dissect these pathological processes. Overall, CNTN3 recombinant protein is a vital resource for advancing neuroscience, disease modeling, and drug discovery, bridging molecular insights with clinical applications.
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