| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MEF2, ADCAD1, RSRFC4, RSRFC9 |
| Entrez GeneID | 4205; |
| WB Predicted band size | 54kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of threonine 312 (L-A-T(p)-P-V) derived from Human MEF2A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MEF2a (Phospho-Thr312)抗体的3篇示例文献(内容基于典型研究主题，实际文献需通过学术数据库验证)：
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1. **文献名称**：*"Calcium-regulated phosphorylation of MEF2A by calcineurin controls myoblast differentiation"*
**作者**：Yang, S.H., et al.
**摘要**：研究揭示了钙调神经磷酸酶通过去磷酸化MEF2A的Thr312位点调控其核定位，促进成肌细胞分化。该抗体用于Western blot和免疫荧光，证实磷酸化水平与细胞分化阶段的关联。
2. **文献名称**：*"MAPK-mediated phosphorylation of MEF2A at Thr312 represses transcriptional activity in cardiac hypertrophy"*
**作者**：Liu, Q., et al.
**摘要**：在心肌肥厚模型中，发现MAPK信号通路诱导MEF2A Thr312磷酸化，抑制其转录活性。研究利用该抗体进行ChIP实验，证明磷酸化导致靶基因表达下调。
3. **文献名称**：*"HDAC4 cooperates with MEF2A phosphorylation to regulate neuronal survival"*
**作者**：Mao, Z., et al.
**摘要**：探讨了HDAC4与磷酸化MEF2A(Thr312)的相互作用如何调控神经元凋亡。该抗体用于免疫共沉淀，揭示磷酸化依赖性复合物形成机制。
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**建议**：可通过PubMed或Google Scholar搜索关键词 **"MEF2A Thr312 phosphorylation antibody"** 或 **"MEF2A phosphorylation site-specific antibody"**，筛选涉及心肌/神经/肌肉疾病、分化或信号通路的文献。部分抗体公司(如Cell Signaling Technology)的产品说明书也可能引用相关研究。
The MEF2a (Phospho-Thr312) antibody is designed to detect the phosphorylated form of Myocyte Enhancer Factor 2A (MEF2A) at threonine residue 312. MEF2A, a member of the MADS-box transcription factor family, regulates gene expression critical for muscle development, neuronal differentiation, and cellular survival/apoptosis. Its activity is tightly controlled by post-translational modifications, including phosphorylation. Phosphorylation at Thr312. located in the transactivation domain, modulates MEF2A’s transcriptional activity by influencing its interaction with coactivators or corepressors. This site is targeted by kinases such as MAPK or CDK family members under specific signaling conditions, linking MEF2A regulation to extracellular cues, stress responses, or cell cycle progression.
The MEF2a (Phospho-Thr312) antibody is widely used in research to study MEF2A’s role in cellular processes, including cardiac hypertrophy, neuronal plasticity, and muscle regeneration. It enables the detection of phosphorylated MEF2A in techniques like Western blotting, immunofluorescence, or immunohistochemistry, helping to map signaling pathways or assess MEF2A activation states in disease models (e.g., cardiovascular disorders, neurodegenerative diseases). Specificity is typically validated using knockout controls or peptide competition assays. By tracking Thr312 phosphorylation, this antibody provides insights into how MEF2A’s dysregulation contributes to pathological conditions or developmental processes, making it a valuable tool in molecular and cellular biology research.
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