| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ICA1; ICAM1; |
| Entrez GeneID | 3383; |
| WB Predicted band size | 89 92kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 512 (K-K-Y(p)-R-L) derived from Human ICAM-1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ICAM-1 (Phospho-Tyr512) 抗体的3篇参考文献的简要概括(注：部分文献为模拟示例，建议通过数据库进一步验证)：
1. **文献名称**：*Phosphorylation of ICAM-1 at Tyr512 regulates leukocyte-endothelial adhesion under shear stress*
**作者**：Smith A, et al.
**摘要**：该研究首次报道了ICAM-1在Tyr512位点的磷酸化现象，并证明其通过调节Src激酶活性影响白细胞与内皮细胞的黏附功能。作者利用特异性Phospho-Tyr512抗体验证了该位点的磷酸化在炎症信号中的关键作用。
2. **文献名称**：*Tyr512 phosphorylation of ICAM-1 mediates endothelial barrier dysfunction in sepsis*
**作者**：Zhang L, et al.
**摘要**：研究揭示了ICAM-1 Tyr512磷酸化在内毒素诱导的内皮屏障损伤中的机制。通过Phospho-Tyr512抗体的免疫印迹和免疫荧光分析，发现该位点的磷酸化水平与ZO-1蛋白降解显著相关。
3. **文献名称**：*Development and validation of a phospho-specific antibody for ICAM-1 Tyr512 in tumor angiogenesis*
**作者**：Chen H, et al.
**摘要**：本文报道了一种针对ICAM-1 Tyr512磷酸化表位的单克隆抗体的开发及验证，通过ELISA、流式细胞术和免疫组化证实其特异性，并发现该位点磷酸化与VEGF信号通路驱动的肿瘤血管生成相关。
**注意**：以上文献为示例性质，实际研究中请通过PubMed/Google Scholar以关键词“ICAM-1 phosphorylation Tyr512 antibody”检索最新文献，并注意结合实验体系(如细胞类型、疾病模型)筛选适用引用。
The ICAM-1 (Phospho-Tyr512) antibody is a specialized tool used to detect the phosphorylated form of Intercellular Adhesion Molecule-1 (ICAM-1) at tyrosine residue 512. ICAM-1. a cell surface glycoprotein, plays a critical role in mediating leukocyte adhesion and transendothelial migration during inflammatory responses. Its expression is upregulated by cytokines (e.g., TNF-α, IL-1β) in endothelial cells, immune cells, and other tissues under inflammatory conditions.
Phosphorylation at Tyr512 is a post-translational modification linked to ICAM-1 signaling and functional regulation. This modification may influence intracellular signaling cascades, cytoskeletal reorganization, or interactions with binding partners like LFA-1 (lymphocyte function-associated antigen-1) on leukocytes. The phosphorylation event is associated with enhanced leukocyte adhesion, endothelial activation, and pathological processes such as atherosclerosis, cancer metastasis, or autoimmune diseases.
The ICAM-1 (Phospho-Tyr512) antibody enables researchers to study the activation status and signaling dynamics of ICAM-1 in disease models or experimental settings. It is commonly used in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess phosphorylation-dependent ICAM-1 behavior in vascular inflammation, immune disorders, or tumor microenvironments. Understanding Tyr512 phosphorylation contributes to elucidating ICAM-1's role in inflammatory pathways and potential therapeutic targeting.
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