| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MYF3; MYOD; MYOD1 |
| Entrez GeneID | 4654; |
| WB Predicted band size | 40kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 200 (A-S-S(p)-P-R) derived from Human MyoD. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MyoD(Phospho-Ser200)抗体的3篇文献示例(注：由于具体文献可能较少，以下内容基于典型研究场景模拟，建议通过数据库验证)：
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1. **文献名称**：*Phosphorylation of MyoD on Serine 200 promotes muscle cell differentiation*
**作者**：Smith J, et al.
**摘要**：该研究报道MyoD在Ser200位点的磷酸化通过调控其与E蛋白的相互作用，增强肌肉特异性基因的转录活性，促进成肌细胞分化。研究使用Phospho-Ser200特异性抗体验证了该位点的磷酸化动力学。
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2. **文献名称**：*MAPK-mediated phosphorylation of MyoD Ser200 modulates its stability during myogenesis*
**作者**：Chen L, et al.
**摘要**：作者发现p38 MAPK通路介导MyoD Ser200磷酸化，延缓其泛素化降解，从而维持MyoD蛋白稳定性。文中通过Phospho-Ser200抗体进行免疫印迹和免疫荧光实验，证实磷酸化水平与肌生成进程相关。
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3. **文献名称**：*Development and validation of a phospho-specific antibody for MyoD Ser200*
**作者**：Rodriguez M, et al.
**摘要**：本文描述了一种针对MyoD Ser200磷酸化位点的多克隆抗体的开发与验证。通过肽段免疫和亲和纯化获得抗体，并在体外激酶实验和肌肉分化模型中验证其特异性，适用于ChIP和Western blot分析。
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**提示**：实际文献需通过PubMed或Google Scholar检索关键词“MyoD Ser200 phosphorylation”或“MyoD Phospho-Ser200 antibody”，并优先选择近5-10年、高引用的研究。部分商业抗体官网(如CST、Abcam)的“参考文献”栏目也可能提供相关论文。
The MyoD (Phospho-Ser200) antibody is a specialized tool used to detect the phosphorylated form of MyoD protein at serine residue 200. MyoD, a key member of the myogenic regulatory factor (MRF) family, is a transcription factor essential for skeletal muscle differentiation and regeneration. It binds to specific DNA sequences to activate muscle-specific gene expression. Post-translational modifications, such as phosphorylation, tightly regulate MyoD’s activity, stability, and interactions with other proteins. Phosphorylation at Ser200 has been implicated in modulating MyoD’s transcriptional activity, potentially influencing its ability to initiate myogenic programs or respond to signaling pathways.
This antibody is commonly employed in research to study the regulatory mechanisms of muscle development, repair, and diseases like muscular dystrophy or cancer cachexia. By targeting the phosphorylated Ser200 epitope, it enables the detection of MyoD’s activated or modified state in techniques such as Western blotting, immunofluorescence, or immunohistochemistry. Specific validation, including knockout controls or peptide competition assays, ensures its specificity for the phosphorylated form. Researchers use it to explore how extracellular signals (e.g., growth factors, stress) or intracellular kinases regulate MyoD during myogenesis, providing insights into muscle biology and therapeutic strategies targeting muscle disorders.
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