| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Bbc2 |
| Entrez GeneID | 12015; |
| WB Predicted band size | 23kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of serine 112 (H-S-S(p)-Y-P) derived from Mouse BAD. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于BAD(Phospho-Ser112)抗体的参考文献示例，基于相关研究领域整理：
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1. **文献名称**: "Regulation of BAD Phosphorylation at Ser112 by the Ras-MAPK Pathway"
**作者**: Zha J. et al.
**摘要**: 研究揭示了Ras-MAPK信号通路通过磷酸化BAD蛋白的Ser112位点抑制其促凋亡功能，并利用特异性抗体证实该修饰在细胞存活调控中的作用。
2. **文献名称**: "AKT Promotes Cell Survival by Phosphorylating BAD at Serine 112"
**作者**: Datta S.R. et al.
**摘要**: 本文证明AKT激酶通过磷酸化BAD的Ser112位点阻断其与Bcl-XL的结合，从而抑制凋亡。研究中使用Phospho-Ser112抗体验证了该修饰在癌症模型中的表达变化。
3. **文献名称**: "Role of BAD Phosphorylation in Glucose Homeostasis and Diabetes"
**作者**: Danial N.N. et al.
**摘要**: 探讨BAD Ser112磷酸化在胰岛β细胞存活中的作用，通过Phospho-Ser112抗体检测发现其磷酸化水平与糖尿病模型中细胞凋亡减少相关。
4. **文献名称**: "Phosphorylation of BAD at Ser112 Mediates Drug Resistance in Leukemia"
**作者**: Kitada S. et al.
**摘要**: 研究发现白血病细胞中BAD Ser112的异常磷酸化与化疗耐药性相关，利用特异性抗体证实其磷酸化水平升高是耐药性的潜在标志物。
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**注**: 以上文献为示例，实际引用需根据具体研究内容核实。建议通过PubMed或Google Scholar以关键词“BAD Phospho-Ser112 antibody”或相关信号通路(如AKT/MAPK)进一步检索。
The BAD (Phospho-Ser112) antibody is a specialized tool used to detect the phosphorylation of the BAD protein at serine residue 112. a critical post-translational modification regulating its function in apoptosis. BAD, a pro-apoptotic member of the Bcl-2 family, promotes cell death by binding and neutralizing anti-apoptotic proteins like Bcl-2 or Bcl-xL. Its activity is tightly controlled by phosphorylation at specific sites, including Ser112. Phosphorylation at Ser112 inactivates BAD by preventing its interaction with Bcl-2 family members, thereby suppressing apoptosis and enhancing cell survival. This modification is mediated by survival-signaling kinases such as AKT, PKA, or RSK, often activated by growth factors or cytokines.
The BAD (Phospho-Ser112) antibody enables researchers to study the activation status of survival pathways in various contexts, including cancer, neurodegeneration, and metabolic disorders. It is widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to assess BAD phosphorylation dynamics under experimental conditions (e.g., drug treatments, stress responses). Validating this antibody’s specificity is crucial, as cross-reactivity with other phospho-epitopes or isoforms may occur. Its application has provided insights into mechanisms of therapeutic resistance, survival signaling crosstalk, and disease-associated apoptotic dysregulation, making it a valuable tool in apoptosis and cell signaling research.
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