| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cytovillin; EZRI; VIL2; Villin 2; p81 |
| Entrez GeneID | 7430; |
| WB Predicted band size | 81kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from Human EZRIN. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Ezrin (Phospho-Tyr353)抗体的参考文献示例(建议通过PubMed或Google Scholar核实具体内容)：
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1. **文献名称**：*"Phosphorylation of Ezrin on Tyrosine 353 Regulates Its Interaction with Rho GTPases"*
**作者**：Ng T. et al.
**摘要**：研究了Ezrin在Tyr353位点的磷酸化如何调控其与Rho家族GTP酶(如RhoA和Cdc42)的相互作用，并影响细胞骨架重组和迁移。
2. **文献名称**：*"Tyrosine 353 Phosphorylation Modulates Ezrin’s Membrane Cytoskeleton Crosslinking Activity"*
**作者**：Fievet B. et al.
**摘要**：通过体外实验证明，Tyr353磷酸化通过改变Ezrin的构象增强其连接细胞膜与肌动蛋白骨架的能力，影响上皮细胞的形态和运动。
3. **文献名称**：*"Ezrin Y353 Phosphorylation as a Biomarker in Breast Cancer Metastasis"*
**作者**：He X. et al.
**摘要**：探讨了Ezrin Tyr353磷酸化在乳腺癌中的临床意义，发现其高表达与肿瘤侵袭性和不良预后相关，可能作为治疗靶点。
4. **文献名称**：*"Phospho-Tyr353-Specific Antibody Reveals Ezrin’s Role in Polarized Cell Migration"*
**作者**：Pujuguet P. et al.
**摘要**：利用Phospho-Tyr353特异性抗体，发现Ezrin磷酸化在细胞极性建立和定向迁移中的关键作用，依赖PI3K信号通路。
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**注意**：上述文献为示例，实际引用时请通过学术数据库(如PubMed、Web of Science)核对作者、标题及摘要准确性。若需具体文献，可搜索关键词“Ezrin phosphorylation Tyr353”或相关信号通路(如Rho GTPases、cancer metastasis)。
**Background of Ezrin (Phospho-Tyr353) Antibody**
Ezrin, a member of the ERM (Ezrin/Radixin/Moesin) protein family, acts as a critical linker between the plasma membrane and the actin cytoskeleton, regulating cell morphology, adhesion, and motility. Its activity is tightly controlled by post-translational modifications, particularly phosphorylation. The phosphorylation of tyrosine 353 (Tyr353) in ezrin plays a pivotal role in modulating its conformational activation. When phosphorylated, ezrin undergoes a structural shift, exposing binding sites that facilitate interactions with membrane-associated proteins and cytoskeletal components.
The Ezrin (Phospho-Tyr353) antibody is a specialized tool designed to detect ezrin specifically phosphorylated at Tyr353. This modification is implicated in signaling pathways linked to cellular processes such as metastasis, epithelial-mesenchymal transition (EMT), and receptor tyrosine kinase signaling. Studies suggest that Tyr353 phosphorylation may enhance ezrin’s role in promoting cancer cell invasion by altering cytoskeletal dynamics or activating downstream effectors.
Researchers utilize this antibody in techniques like Western blotting, immunofluorescence, or immunohistochemistry to investigate ezrin activation states in pathological contexts, including cancer progression or immune cell regulation. Its specificity helps dissect the functional relevance of ezrin phosphorylation in disease models, offering insights into potential therapeutic targets. Validation of the antibody includes testing in cell lines with known phosphorylation status or genetic modifications to ensure accuracy. Understanding ezrin’s phospho-regulation at Tyr353 remains vital for unraveling its contributions to cellular plasticity and disease mechanisms.
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