Rabbit Polyclonal PKD/PKCm(Phospho-Ser910) Antibody

The PKD/PKCμ (Phospho-Ser910) antibody is a specialized tool used to detect the activated form of Protein Kinase D (PKD), also known as PKCμ, phosphorylated at serine residue 910. PKD belongs to the calcium/calmodulin-dependent kinase superfamily and plays a critical role in cellular signaling pathways regulating processes like proliferation, apoptosis, migration, and vesicle trafficking. Phosphorylation at Ser910 is a key post-translational modification associated with PKD activation, often occurring downstream of G protein-coupled receptors (GPCRs) or receptor tyrosine kinases (RTKs). This phosphorylation event is mediated by PKC isoforms, which trigger PKD’s catalytic activity by relieving autoinhibition. The antibody specifically recognizes this phosphorylated state, enabling researchers to study PKD activation dynamics in response to stimuli such as growth factors, hormones, or stress signals. It is widely utilized in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate PKD’s role in diseases, including cancer (e.g., tumor invasion, angiogenesis), cardiovascular disorders, and inflammatory conditions. Validated applications often include correlating Ser910 phosphorylation with downstream targets like HDAC5 or MAPK pathways. Proper experimental controls, such as kinase inhibition or siRNA knockdown, are recommended to confirm specificity.