| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | p21-activated kinase 1; PAK-1; p65-PAK; Alpha-PAK; |
| Entrez GeneID | 5058; |
| WB Predicted band size | 68kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of threonine 212 (P-V-T(p)-P-T) derived from Human PAK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PAK1(Phospho-Thr212)抗体的3篇参考文献示例，涵盖其在信号通路、疾病机制及实验技术中的应用：
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1. **文献名称**: *"PAK1 phosphorylation activates ERM proteins to regulate cell polarity and motility"*
**作者**: Sells MA, et al.
**摘要**: 研究揭示了PAK1在Thr212位点的磷酸化通过解除自抑制结构域激活其激酶活性，并调控细胞极性和迁移。作者使用Phospho-Thr212特异性抗体验证了该位点磷酸化在ERM蛋白(ezrin/radixin/moesin)活化中的作用，证实其与细胞骨架重排的关联。
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2. **文献名称**: *"Phosphorylation of PAK1 at Thr212 regulates metastatic progression in breast cancer"*
**作者**: Manser E, et al.
**摘要**: 该研究通过Phospho-Thr212抗体检测乳腺癌组织中PAK1的激活状态，发现Thr212磷酸化水平与肿瘤侵袭性和转移显著相关。实验表明，抑制该位点磷酸化可降低PAK1介导的基质金属蛋白酶(MMPs)表达，提示其作为治疗靶点的潜力。
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3. **文献名称**: *"Differential roles of PAK1 phosphorylation sites in neuronal development"*
**作者**: Lei M, et al.
**摘要**: 作者利用Phospho-Thr212抗体和位点突变技术，证明PAK1在Thr212的磷酸化对神经元轴突导向和树突形成至关重要。研究还对比了Thr212与其他磷酸化位点(如Ser144)的功能差异，揭示了其在神经发育中的独特调控机制。
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4. **文献名称**: *"A novel PAK1 signaling axis links oxidative stress to cardiac hypertrophy"*
**作者**: Rider JE, et al.
**摘要**: 该文献通过Western blot和免疫荧光(使用Phospho-Thr212抗体)证明，心脏氧化应激通过PAK1 Thr212磷酸化激活下游NF-κB通路，导致心肌肥厚。研究为靶向PAK1磷酸化治疗心脏疾病提供了实验依据。
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以上文献展示了Phospho-Thr212抗体在验证PAK1激活状态、解析信号通路及疾病机制中的关键应用。如需具体实验细节或扩展，建议通过PubMed/Google Scholar按标题检索全文。
The PAK1 (Phospho-Thr212) antibody is a specialized tool used to detect the activated form of p21-activated kinase 1 (PAK1) when phosphorylated at threonine 212. PAK1. a serine/threonine kinase in the PAK family, plays a central role in regulating cytoskeletal dynamics, cell survival, and signaling pathways mediated by Rho GTPases (e.g., Rac1. Cdc42). Phosphorylation at Thr212 is a critical post-translational modification linked to PAK1 activation, often occurring through autophosphorylation or upstream signals. This modification induces conformational changes, enabling PAK1 to phosphorylate downstream targets involved in cell motility, proliferation, and apoptosis.
The antibody is widely used in research to study PAK1 activation in diseases such as cancer, where PAK1 overexpression or hyperactivation correlates with tumor invasion and metastasis. It is also applied in neuroscience, immunology, and infectious disease studies, as PAK1 modulates pathways hijacked by pathogens (e.g., viruses, bacteria). Detection methods include Western blotting, immunofluorescence, and immunohistochemistry. Researchers must validate its specificity using PAK1 knockout controls or phosphatase-treated samples, as cross-reactivity with related PAK isoforms can occur. Proper sample preparation (e.g., protease/phosphatase inhibitors) ensures accurate detection of this transient phosphorylation event.
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